Hexokinase activity and aging.
Bone marrow mesenchymal stem
cells (MSC) were cultured in vitro.
Early and late passage MSC were
harvested for enzyme assay. The
study shows that hexokinase
activity is increased upon repeated
cell passages in vitro.
Welcome to BMR
- Streamlining Laboratory Medicine -

Biomedical Research Service & Clinical
Application is affiliated with the School of
Medicine and Biomedical Sciences,
University at Buffalo. The center produces
high-quality, cost-effective research and
diagnostic reagents for the broad life
science and clinical communities, and
promotes collaborative translational
research. Please send your inquiry to:

Dr. Techung Lee
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Email: chunglee@buffalo.edu
Tel: (716) 829-3106
An easy assay of fatty acid
capacity. Mice were fed
methionine-choline deficient diet
(MCDD) for 14 weeks. Heart tissues
were homogenized and FAO
activities were assayed by the FAO
Assay kit.
Biomedical Research Service
& Clinical Application
An enzyme marker of clinical utility.
Mice were fed methionine-choline
deficient diet (MCDD) for 6 weeks.
Liver tissues were homogenized
and PGI activities were assayed by
the PGI Assay kit.
Cell Regulation
Cell Viability
Cell Injury
Cell Proliferation
DPP-4 (CD26) Assay
Alkaline Phosphatase Assay
Matrix Metalloproteinase
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Useful websites for biomedical research

Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed

Nucleic Acid/Protein Analysis by Colorado State Univ- http://www.vivo.colostate.edu/molkit/index.html

Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3

Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH

US National Library of Medicine- http://www.ncbi.nlm.nih.gov/pubmed   

US National Institute of Health- http://www.nih.gov   

NIH Clinical Trials- http://clinicaltrials.gov   

NIH Gene Therapy Program- http://www.gtrp.org                                                                                                                                       

US Patent Office- http://www.uspto.gov

Knockout Mouse Project by UC Davis- http://www.komp.org

Shared Facilities of Roswell Park Cancer Institute- http://www.roswellpark.edu/shared-resources

Shared Facilities of University at Buffalo- http://medicine.buffalo.edu/research/core_facilities.html

American Type Culture Collection- http://www.atcc.org

Sigma MSDS Search- http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do

Histology information by Histonet- http://www.histonet.org
Energy Metabolism
Hexokinase (HK)
Phosphoglucoisomerase (PGI)
GAP dehydrogenase (GAPDH)
Lactate dehydrogenase (LDH)
Pyruvate dehydrogenase (PDH)
Malate dehydrogenase (MDH)
Fatty acid oxidation (FAO)
Pyruvate Kinase (PK)
Creatine Kinase (CK)
Adenylate Kinase (ADK)
Sorbitol Dehydrogenase (SDH)
Amino Acid/Lipid/Nucleotide Metabolism
Glutamate Dehydrogenase (GLDH) Assay
Alanine Aminotransferase (ALT or GPT) Assay
Aspartate Aminotransferase (AST or GOT) Assay
Threonine Dehydrogenase Assay
Inosine-5′-monophosphate Dehydrogenase (IMPDH) Assay
Nucleoside Diphosphate Kinase (NDK) Assay
Glucose-6-phosphate dehydrogenase (G6PD)  
Adenylate Kinase (ADK or myokinase) Assay
Glycerol-3-phosphate dehydrogenase (GPDH) Assay
G6P dehydrogenase (G6PD) Assay
Glutamate Assay
Glutaminase Assay
Tyrosine Aminotransferase (TAT) Assay
GABA Aminotransferase (GABAT) Assay
Detection Systems         
TMB Substrate kit
DAB Substrate kit
BCIP Substrate kit
Alkaline Phosphatase
Horseradish Peroxidase


Viral Vectors

cDNA clones
Ethanol Metabolism      
Alcohol dehydrogenase (ADH)
Aldehyde dehydrogenase (ALDH)
Stress Response
Lipid Peroxidation
Cell Death
Nitric Oxide
Intracellular Thiols
Protease Activation
Catalase Assay
Glutathione Assay
GST Assay
Senescence Detection
Here are some of the
pharmaceutical and biotech
companies we have proudly
served over the years:
PGI Assay
Fatty Acid Oxidation
Hexokinase Assay
Discovering IMPDH inhibitors.
HeLa cells were treated with DMSO
(control) and 10 uM mycophenolic
acid (MPA) for 24 hours, following
which cells were harvested and
IMPDH activity was measured
using the IMPDH assay kit.
MMP2/9 Assay
Discovering inhibitor of MMP2/9.
Prinomastat, a potent MMP
inhibitor, reduces the activity of
MMP2/9 by ~70% at 2.5 nM using
the assay kit.