Bone marrow mesenchymal
stem cells (MSC) were
cultured in vitro. Early and
late passage MSC were
harvested for enzyme assay.
The study shows that
hexokinase activity is
increased during cellular
senescence.
stem cells (MSC) were
cultured in vitro. Early and
late passage MSC were
harvested for enzyme assay.
The study shows that
hexokinase activity is
increased during cellular
senescence.
Welcome to BMR
- Streamlining Laboratory Medicine -
Biomedical Research Service & Clinical
Application is affiliated with the School of
Medicine and Biomedical Sciences,
University at Buffalo. The center produces
high-quality, cost-effective research and
diagnostic reagents for the broad life
science and clinical communities, and
promotes collaborative translational
research. Please send your inquiry to:
Dr. Techung Lee
Director
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Email: chunglee@buffalo.edu
Tel: (716) 829-3106
- Streamlining Laboratory Medicine -
Biomedical Research Service & Clinical
Application is affiliated with the School of
Medicine and Biomedical Sciences,
University at Buffalo. The center produces
high-quality, cost-effective research and
diagnostic reagents for the broad life
science and clinical communities, and
promotes collaborative translational
research. Please send your inquiry to:
Dr. Techung Lee
Director
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Email: chunglee@buffalo.edu
Tel: (716) 829-3106
Mice were fed methionine- and
choline-deficient (MCD) diet for
14 weeks. Heart tissues were
homogenized and FAO
activities were assayed. The
data indicate that cardiac fatty
acid oxidation capacity can be
compromised in the fatty acid
model.
choline-deficient (MCD) diet for
14 weeks. Heart tissues were
homogenized and FAO
activities were assayed. The
data indicate that cardiac fatty
acid oxidation capacity can be
compromised in the fatty acid
model.
Biomedical Research Service
& Clinical Application
& Clinical Application
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Mice were fed methionine- and
choline-deficient (MCD) diet for 6
weeks. Liver tissues were
homogenized and PGI activities
were assayed. The data show that
the fatty liver exhibits increased
PGI activities. In addition, the
serum level of PGI activity is also
increased in the fatty liver model.
choline-deficient (MCD) diet for 6
weeks. Liver tissues were
homogenized and PGI activities
were assayed. The data show that
the fatty liver exhibits increased
PGI activities. In addition, the
serum level of PGI activity is also
increased in the fatty liver model.
Cell Regulation
O-GlcNAcylation
Nagalase Assay
Cell Viability
Cell Proliferation
DPP-4 (CD26) Assay
Osteogenesis
Chondrogenesis
Adipogenesis
Alkaline Phosphatase
Matrix Metalloproteinase
O-GlcNAcylation
Nagalase Assay
Cell Viability
Cell Proliferation
DPP-4 (CD26) Assay
Osteogenesis
Chondrogenesis
Adipogenesis
Alkaline Phosphatase
Matrix Metalloproteinase
Useful websites for biomedical research
Science Job- http://jobs.sciencecareers.org/jobs
Nature Job- http://www.nature.com/naturejobs/science/welcome
Monster Job- http://www.monster.com
Google Job-http://www.google.com/about/jobs/?gclid=CJ_Iyp7_iLsCFe87MgodSA0AjQ#src=Online/House%20Ads/BKWS&utm_source=adwords&utm_medium=text&utm_campaign=BKWS
Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed
Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3
Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH
US National Library of Medicine- http://www.ncbi.nlm.nih.gov/pubmed
US National Institute of Health- http://www.nih.gov
NIH Clinical Trials- http://clinicaltrials.gov
NIH Gene Therapy Program- http://www.gtrp.org
US Patent Office- http://www.uspto.gov
Knockout Mouse Project by UC Davis- http://www.komp.org
Shared Facilities of Roswell Park Cancer Institute- http://www.roswellpark.edu/shared-resources
Shared Facilities of University at Buffalo- http://medicine.buffalo.edu/research/core_facilities.html
American Type Culture Collection- http://www.atcc.org
Sigma MSDS Search- http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Science Job- http://jobs.sciencecareers.org/jobs
Nature Job- http://www.nature.com/naturejobs/science/welcome
Monster Job- http://www.monster.com
Google Job-http://www.google.com/about/jobs/?gclid=CJ_Iyp7_iLsCFe87MgodSA0AjQ#src=Online/House%20Ads/BKWS&utm_source=adwords&utm_medium=text&utm_campaign=BKWS
Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed
Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3
Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH
US National Library of Medicine- http://www.ncbi.nlm.nih.gov/pubmed
US National Institute of Health- http://www.nih.gov
NIH Clinical Trials- http://clinicaltrials.gov
NIH Gene Therapy Program- http://www.gtrp.org
US Patent Office- http://www.uspto.gov
Knockout Mouse Project by UC Davis- http://www.komp.org
Shared Facilities of Roswell Park Cancer Institute- http://www.roswellpark.edu/shared-resources
Shared Facilities of University at Buffalo- http://medicine.buffalo.edu/research/core_facilities.html
American Type Culture Collection- http://www.atcc.org
Sigma MSDS Search- http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Energy Metabolism
Hexokinase (HK)
Phosphoglucoisomerase (PGI)
GAP dehydrogenase (GAPDH)
Lactate dehydrogenase (LDH)
Pyruvate dehydrogenase (PDH)
Malate dehydrogenase (MDH)
Fatty acid oxidation (FAO)
Pyruvate Kinase (PK)
Creatine Kinase (CK)
Adenylate Kinase (ADK)
Sorbitol Dehydrogenase (SDH)
L-lactate
D-lactate
ATP/ADP/AMP
Pyruvate
NAD+
Glucose
Glucose-6-phosphate
Hexokinase (HK)
Phosphoglucoisomerase (PGI)
GAP dehydrogenase (GAPDH)
Lactate dehydrogenase (LDH)
Pyruvate dehydrogenase (PDH)
Malate dehydrogenase (MDH)
Fatty acid oxidation (FAO)
Pyruvate Kinase (PK)
Creatine Kinase (CK)
Adenylate Kinase (ADK)
Sorbitol Dehydrogenase (SDH)
L-lactate
D-lactate
ATP/ADP/AMP
Pyruvate
NAD+
Glucose
Glucose-6-phosphate
Amino Acid/Lipid/Nucleotide Metabolism
Glutamate Dehydrogenase (GLDH) Assay
Alanine Aminotransferase (ALT or GPT) Assay
Aspartate Aminotransferase (AST or GOT) Assay
Threonine Dehydrogenase Assay
Inosine-5′-monophosphate Dehydrogenase (IMPDH) Assay
Nucleoside Diphosphate Kinase (NDK) Assay
Glucose-6-phosphate dehydrogenase (G6PD)
Adenylate Kinase (ADK or myokinase) Assay
Glycerol-3-phosphate dehydrogenase (GPDH) Assay
G6P dehydrogenase (G6PD) Assay
Glutamate Assay
Glutaminase Assay
Tyrosine Aminotransferase (TAT) Assay
GABA Aminotransferase (GABAT) Assay
Glutamate Dehydrogenase (GLDH) Assay
Alanine Aminotransferase (ALT or GPT) Assay
Aspartate Aminotransferase (AST or GOT) Assay
Threonine Dehydrogenase Assay
Inosine-5′-monophosphate Dehydrogenase (IMPDH) Assay
Nucleoside Diphosphate Kinase (NDK) Assay
Glucose-6-phosphate dehydrogenase (G6PD)
Adenylate Kinase (ADK or myokinase) Assay
Glycerol-3-phosphate dehydrogenase (GPDH) Assay
G6P dehydrogenase (G6PD) Assay
Glutamate Assay
Glutaminase Assay
Tyrosine Aminotransferase (TAT) Assay
GABA Aminotransferase (GABAT) Assay
Detection Systems
TMB Substrate kit
DAB Substrate kit
BCIP Substrate kit
Alkaline Phosphatase
Horseradish Peroxidase
Luciferase
LacZ
Proteins/Antibodies
Viral Vectors
cDNA clones
TMB Substrate kit
DAB Substrate kit
BCIP Substrate kit
Alkaline Phosphatase
Horseradish Peroxidase
Luciferase
LacZ
Proteins/Antibodies
Viral Vectors
cDNA clones
Ethanol Metabolism
Alcohol dehydrogenase (ADH)
Aldehyde dehydrogenase (ALDH)
Ethanol
Alcohol dehydrogenase (ADH)
Aldehyde dehydrogenase (ALDH)
Ethanol
Stress Response
Peroxide
Lipid Peroxidation
Cell Death
Nitric Oxide
Intracellular Thiols
Catalase Assay
Glutathione Assay
GST Assay
Senescence Detection
Peroxide
Lipid Peroxidation
Cell Death
Nitric Oxide
Intracellular Thiols
Catalase Assay
Glutathione Assay
GST Assay
Senescence Detection
Here are some of the pharmaceutical and biotech
companies we have served over the years:
companies we have served over the years:
HeLa cells were treated with
DMSO (control) and 10 uM
mycophenolic acid (MPA) for
24 hours, following which cells
were harvested and IMPDH
activity was measured. The
IMPDH activity assay kit has
been used for drug discovery
application.
DMSO (control) and 10 uM
mycophenolic acid (MPA) for
24 hours, following which cells
were harvested and IMPDH
activity was measured. The
IMPDH activity assay kit has
been used for drug discovery
application.
Matrix metalloproteinases (MMPs)
play important roles in ECM
remodeling. Prinomastat, a potent
MMP inhibitor, reduces the activity
of MMP2/9 by ~70% at 2.5 nM
using the convenient MMP2/9
liquid assay kit.
play important roles in ECM
remodeling. Prinomastat, a potent
MMP inhibitor, reduces the activity
of MMP2/9 by ~70% at 2.5 nM
using the convenient MMP2/9
liquid assay kit.
Skeletal muscle tissues were
collected from normal
hamster (F1B strain) and
dystrophic strain (TO2), and
homogenized. Muslce
Nagalase enzyme activity
was measured, showing that
muscular dystrophy is
associated with increased
Nagalase enzyme activity.
collected from normal
hamster (F1B strain) and
dystrophic strain (TO2), and
homogenized. Muslce
Nagalase enzyme activity
was measured, showing that
muscular dystrophy is
associated with increased
Nagalase enzyme activity.