Measuring the activities of alkaline
phosphatase (Alk) and beta-galactosidase
(Gal) in crude cell extracts. Early and late
passage bone marrow mesenchymal stem
cells are solubilized in a lysis buffer, and
cell lysates are mixed with Alk and Gal
assay solutions. The assays demonstrate
differential changes in enzyme activities
during stem cell senescence in culture.
phosphatase (Alk) and beta-galactosidase
(Gal) in crude cell extracts. Early and late
passage bone marrow mesenchymal stem
cells are solubilized in a lysis buffer, and
cell lysates are mixed with Alk and Gal
assay solutions. The assays demonstrate
differential changes in enzyme activities
during stem cell senescence in culture.
Welcome to
Biomedical Research Service
& Clinical Application
- Streamlining Laboratory Medicine
Biomedical Research Service & Clinical Application
(BRSC) is affiliated with the School of Medicine and
Biomedical Sciences, University at Buffalo. The
center manufactures high-quality, cost-effective
research and diagnostic reagents for the broad life
science and clinical communities, and promotes
collaborative translational research. Please send
your inquiry to:
Dr. Techung Lee
Director of BRSC
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Biomedical Research Service
& Clinical Application
- Streamlining Laboratory Medicine
Biomedical Research Service & Clinical Application
(BRSC) is affiliated with the School of Medicine and
Biomedical Sciences, University at Buffalo. The
center manufactures high-quality, cost-effective
research and diagnostic reagents for the broad life
science and clinical communities, and promotes
collaborative translational research. Please send
your inquiry to:
Dr. Techung Lee
Director of BRSC
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Measuring ethanol content in Red Beaujolais
Wine using our Ethanol Assay kit. The Ethanol
Assay Solution is mixed with 50 ul of 200-fold
diluted Red Wine, and the mixture is incubated
for 30 min. O.D.492 nm is measured, and
ethanol concentration is deduced from an
ethanol standard plot. The assay shows that the
Red Wine contains ~13% alcohol.
Wine using our Ethanol Assay kit. The Ethanol
Assay Solution is mixed with 50 ul of 200-fold
diluted Red Wine, and the mixture is incubated
for 30 min. O.D.492 nm is measured, and
ethanol concentration is deduced from an
ethanol standard plot. The assay shows that the
Red Wine contains ~13% alcohol.
Biomedical Research Service
& Clinical Application
& Clinical Application
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Versatile ethanol assay
Popular L-lactate assay
Measuring L-lactate levels in culture
media. HEK293 cells constitutively
expressing bcl-2 show altered glucose
metabolism. Culture media are diluted 10
fold and mixed with the Lactate Assay
Solution. L-lactate concentrations are
deduced from a lactate standard plot. The
assay reveals that lactate production by
the Bcl-2 expressor is reduced compared
to the parental HEK293 cells.
media. HEK293 cells constitutively
expressing bcl-2 show altered glucose
metabolism. Culture media are diluted 10
fold and mixed with the Lactate Assay
Solution. L-lactate concentrations are
deduced from a lactate standard plot. The
assay reveals that lactate production by
the Bcl-2 expressor is reduced compared
to the parental HEK293 cells.
Rapid purification of plasmid DNA for DNA
sequencing and molecular cloning. Plasmid
DNA is isolated using our simple and
inexpensive Plasmid DNA Purification kit.
Each sequencing run can generate up to ~1kb
sequence information.
sequencing and molecular cloning. Plasmid
DNA is isolated using our simple and
inexpensive Plasmid DNA Purification kit.
Each sequencing run can generate up to ~1kb
sequence information.
Streamlined plasmid DNA purification
One-step enzyme measurements
Sensitive detection of serum D-lactate
Measuring D-lactate levels in human
serum. Less than 0.05 ml of human
serum is required for the detection. The
sensitive D-lactate assay can detect as
low as 10 uM D-lactate present in serum
in ~30 min.
serum. Less than 0.05 ml of human
serum is required for the detection. The
sensitive D-lactate assay can detect as
low as 10 uM D-lactate present in serum
in ~30 min.
Fuel Metabolism Ethanol Metabolism Detection Systems
Hexokinase (HK) Alcohol dehydrogenase ELISA Detection System (TMB)
GAP dehydrogenase (GAPDH) Aldehyde dehydrogenase Enhanced Chemiluminescence (Luminol)
Lactate dehydrogenase (LDH) Ethanol Alkaline phosphatase
Glucose-6-phosphate dehydrogenase (G6PD) Luciferase
Pyruvate dehydrogenase (PDH) LacZ
Malate dehydrogenase (MDH) Cell Assays
Pyruvate Kinase (PK) Cell Viability/Proliferation Stress Response
L-lactate Cell Injury Peroxide
D-lactate Osteogenesis Lipid Peroxidation
ATP Chondrogenesis Cell Death
Pyruvate Adipogenesis Nitric Oxide
NAD+
Glucose
Amino Acid Metabolism
L-Glutamate Assay
Glutamate Dehydrogenase (GLDH)
Alanine Aminotransferase (ALT or GPT)
Aspartate Aminotransferase (AST or GOT)
Hexokinase (HK) Alcohol dehydrogenase ELISA Detection System (TMB)
GAP dehydrogenase (GAPDH) Aldehyde dehydrogenase Enhanced Chemiluminescence (Luminol)
Lactate dehydrogenase (LDH) Ethanol Alkaline phosphatase
Glucose-6-phosphate dehydrogenase (G6PD) Luciferase
Pyruvate dehydrogenase (PDH) LacZ
Malate dehydrogenase (MDH) Cell Assays
Pyruvate Kinase (PK) Cell Viability/Proliferation Stress Response
L-lactate Cell Injury Peroxide
D-lactate Osteogenesis Lipid Peroxidation
ATP Chondrogenesis Cell Death
Pyruvate Adipogenesis Nitric Oxide
NAD+
Glucose
Amino Acid Metabolism
L-Glutamate Assay
Glutamate Dehydrogenase (GLDH)
Alanine Aminotransferase (ALT or GPT)
Aspartate Aminotransferase (AST or GOT)
Connection Web Sites
Scheduling- http://www.doodle.com
Web Conferencing- http://www.webex.com
Web Conferencing- http://www.gotomeeting.com/fec
Lab Meeting- http://mylabmeetings.com/group_meetings
Scheduling- http://www.doodle.com
Web Conferencing- http://www.webex.com
Web Conferencing- http://www.gotomeeting.com/fec
Lab Meeting- http://mylabmeetings.com/group_meetings
Job Search Web Sites
Science Job- http://scjobs.sciencemag.org/JobSeekerX
Nature Job- http://www.nature.com/naturejobs/science/welcome
Monster Job- http://www.monster.com
Google Job- http://www.google.com/Top/Science/Biology/Employment/Job_Search
Science Job- http://scjobs.sciencemag.org/JobSeekerX
Nature Job- http://www.nature.com/naturejobs/science/welcome
Monster Job- http://www.monster.com
Google Job- http://www.google.com/Top/Science/Biology/Employment/Job_Search
Research Tool Web Sites
Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed
Nucleic Acid/Protein Analysis by Colorado State Univ- http://www.vivo.colostate.edu/molkit/index.html
Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3
Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH
Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed
Nucleic Acid/Protein Analysis by Colorado State Univ- http://www.vivo.colostate.edu/molkit/index.html
Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3
Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH
