Measuring hexokinase activity in
cellular aging. Bone marrow
mesenchymal stem cells (MSC)
were cultured in vitro. Early and
late passage MSC were harvested
for enzyme assay. The study
shows that hexokinase activity is
increased upon repeated cell
passages in vitro.
cellular aging. Bone marrow
mesenchymal stem cells (MSC)
were cultured in vitro. Early and
late passage MSC were harvested
for enzyme assay. The study
shows that hexokinase activity is
increased upon repeated cell
passages in vitro.
Welcome to BMR
- Streamlining Laboratory Medicine -
Biomedical Research Service & Clinical
Application is affiliated with the School of
Medicine and Biomedical Sciences,
University at Buffalo. The center produces
high-quality, cost-effective research and
diagnostic reagents for the broad life
science and clinical communities, and
promotes collaborative translational
research. Please send your inquiry to:
Dr. Techung Lee
Director
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Email: chunglee@buffalo.edu
Tel: (716) 829-3106
- Streamlining Laboratory Medicine -
Biomedical Research Service & Clinical
Application is affiliated with the School of
Medicine and Biomedical Sciences,
University at Buffalo. The center produces
high-quality, cost-effective research and
diagnostic reagents for the broad life
science and clinical communities, and
promotes collaborative translational
research. Please send your inquiry to:
Dr. Techung Lee
Director
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Email: chunglee@buffalo.edu
Tel: (716) 829-3106
Measuring ethanol content in Red
Beaujolais Wine. The one-step
assay shows that the Red Wine
contains ~13% alcohol.
Beaujolais Wine. The one-step
assay shows that the Red Wine
contains ~13% alcohol.
Biomedical Research Service
& Clinical Application
& Clinical Application
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Measuring L-lactate levels in
culture media. The assay reveals
that lactate production by the Bcl-2
expressor is reduced compared to
the parental HEK293 cells.
culture media. The assay reveals
that lactate production by the Bcl-2
expressor is reduced compared to
the parental HEK293 cells.
Cell Regulation
O-GlcNAcylation
Cell Viability
Cell Injury
Cell Proliferation
DPP-4 (CD26) Assay
Osteogenesis
Chondrogenesis
Adipogenesis
Alkaline Phosphatase Assay
Matrix Metalloproteinase
O-GlcNAcylation
Cell Viability
Cell Injury
Cell Proliferation
DPP-4 (CD26) Assay
Osteogenesis
Chondrogenesis
Adipogenesis
Alkaline Phosphatase Assay
Matrix Metalloproteinase
Scheduling- http://www.doodle.com
Web Conferencing- http://www.webex.com
Web Conferencing- http://www.gotomeeting.com/fec
Lab Meeting- http://mylabmeetings.com/group_meetings
Science Job- http://jobs.sciencecareers.org/jobs
Nature Job- http://www.nature.com/naturejobs/science/welcome
Monster Job- http://www.monster.com
Google Job-http://www.google.com/about/jobs/?gclid=CJ_Iyp7_iLsCFe87MgodSA0AjQ#src=Online/House%20Ads/BKWS&utm_source=adwords&utm_medium=text&utm_campaign=BKWS
Useful websites for biomedical research
Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed
Nucleic Acid/Protein Analysis by Colorado State Univ- http://www.vivo.colostate.edu/molkit/index.html
Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3
Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH
US National Library of Medicine- http://www.ncbi.nlm.nih.gov/pubmed
US National Institute of Health- http://www.nih.gov
NIH Clinical Trials- http://clinicaltrials.gov
NIH Gene Therapy Program- http://www.gtrp.org
US Patent Office- http://www.uspto.gov
Knockout Mouse Project by UC Davis- http://www.komp.org
Shared Facilities of Roswell Park Cancer Institute- http://www.roswellpark.edu/shared-resources
Shared Facilities of University at Buffalo- http://medicine.buffalo.edu/research/core_facilities.html
American Type Culture Collection- http://www.atcc.org
Sigma MSDS Search- http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Histology information by Histonet- http://www.histonet.org
Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed
Nucleic Acid/Protein Analysis by Colorado State Univ- http://www.vivo.colostate.edu/molkit/index.html
Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3
Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH
US National Library of Medicine- http://www.ncbi.nlm.nih.gov/pubmed
US National Institute of Health- http://www.nih.gov
NIH Clinical Trials- http://clinicaltrials.gov
NIH Gene Therapy Program- http://www.gtrp.org
US Patent Office- http://www.uspto.gov
Knockout Mouse Project by UC Davis- http://www.komp.org
Shared Facilities of Roswell Park Cancer Institute- http://www.roswellpark.edu/shared-resources
Shared Facilities of University at Buffalo- http://medicine.buffalo.edu/research/core_facilities.html
American Type Culture Collection- http://www.atcc.org
Sigma MSDS Search- http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Histology information by Histonet- http://www.histonet.org
Energy Metabolism
Isocitrate dehydrogenase (IDH)
Hexokinase (HK)
GAP dehydrogenase (GAPDH)
Lactate dehydrogenase (LDH)
Pyruvate dehydrogenase (PDH)
Malate dehydrogenase (MDH)
Pyruvate Kinase (PK)
Creatine Kinase (CK)
Adenylate Kinase (ADK)
Sorbitol Dehydrogenase (SDH)
L-lactate
D-lactate
ATP/ADP/AMP
Pyruvate
NAD+
Glucose
Glucose-6-phosphate
Isocitrate dehydrogenase (IDH)
Hexokinase (HK)
GAP dehydrogenase (GAPDH)
Lactate dehydrogenase (LDH)
Pyruvate dehydrogenase (PDH)
Malate dehydrogenase (MDH)
Pyruvate Kinase (PK)
Creatine Kinase (CK)
Adenylate Kinase (ADK)
Sorbitol Dehydrogenase (SDH)
L-lactate
D-lactate
ATP/ADP/AMP
Pyruvate
NAD+
Glucose
Glucose-6-phosphate
Amino Acid/Lipid/Nucleotide Metabolism
Glutamate Dehydrogenase (GLDH) Assay
Alanine Aminotransferase (ALT or GPT) Assay
Aspartate Aminotransferase (AST or GOT) Assay
Threonine Dehydrogenase Assay
Inosine-5′-monophosphate Dehydrogenase (IMPDH) Assay
Nucleoside Diphosphate Kinase (NDK) Assay
Glucose-6-phosphate dehydrogenase (G6PD)
Adenylate Kinase (ADK or myokinase) Assay
Glycerol-3-phosphate dehydrogenase (GPDH) Assay
G6P dehydrogenase (G6PD) Assay
Glutamate Assay
Glutaminase Assay
Tyrosine Aminotransferase (TAT) Assay
GABA Aminotransferase (GABAT) Assay
Glutamate Dehydrogenase (GLDH) Assay
Alanine Aminotransferase (ALT or GPT) Assay
Aspartate Aminotransferase (AST or GOT) Assay
Threonine Dehydrogenase Assay
Inosine-5′-monophosphate Dehydrogenase (IMPDH) Assay
Nucleoside Diphosphate Kinase (NDK) Assay
Glucose-6-phosphate dehydrogenase (G6PD)
Adenylate Kinase (ADK or myokinase) Assay
Glycerol-3-phosphate dehydrogenase (GPDH) Assay
G6P dehydrogenase (G6PD) Assay
Glutamate Assay
Glutaminase Assay
Tyrosine Aminotransferase (TAT) Assay
GABA Aminotransferase (GABAT) Assay
Detection Systems
TMB Substrate kit
DAB Substrate kit
BCIP Substrate kit
Alkaline Phosphatase
Horseradish Peroxidase
Luciferase
LacZ
Proteins/Antibodies
Viral Vectors
cDNA clones
TMB Substrate kit
DAB Substrate kit
BCIP Substrate kit
Alkaline Phosphatase
Horseradish Peroxidase
Luciferase
LacZ
Proteins/Antibodies
Viral Vectors
cDNA clones
Ethanol Metabolism
Alcohol dehydrogenase (ADH)
Aldehyde dehydrogenase (ALDH)
Ethanol
Alcohol dehydrogenase (ADH)
Aldehyde dehydrogenase (ALDH)
Ethanol
Stress Response
Peroxide
Lipid Peroxidation
Cell Death
Nitric Oxide
Intracellular Thiols
Protease Activation
Catalase Assay
Glutathione Assay
GST Assay
Senescence Detection
Peroxide
Lipid Peroxidation
Cell Death
Nitric Oxide
Intracellular Thiols
Protease Activation
Catalase Assay
Glutathione Assay
GST Assay
Senescence Detection
Here are some of the
pharmaceutical and biotech
companies we have proudly
served over the years:
pharmaceutical and biotech
companies we have proudly
served over the years:
L-Lactate Assay
L-Lactate Assay
Discovering IMPDH inhibitors.
HeLa cells were treated with
DMSO (control) and 10 uM
mycophenolic acid (MPA) for 24
hours, following which cells were
harvested and IMPDH activity was
measured using the IMPDH assay
kit.
HeLa cells were treated with
DMSO (control) and 10 uM
mycophenolic acid (MPA) for 24
hours, following which cells were
harvested and IMPDH activity was
measured using the IMPDH assay
kit.
Discovering inhibitor of MMP2/9.
Prinomastat, a potent MMP
inhibitor, reduces the activity of
MMP2/9 by ~70% at 2.5 nM using
the assay kit.
Prinomastat, a potent MMP
inhibitor, reduces the activity of
MMP2/9 by ~70% at 2.5 nM using
the assay kit.