Aldehyde Dehydrogenase (ALDH) Assay Kit Price
Product Description: In mammals, ethanol is metabolized mainly in the liver by alcohol dehydrogenase (ADH), which oxidizes ethanol to
acetaldehyde. Acetaldehyde, a toxic metabolite responsible for the miserable effects of hangovers, is further oxidized to acetate by aldehyde
dehydrogenase (ALDH). ALDH belongs to a large family of aldehyde dehydrogenases that can be found in many tissues of the body, but are at
the highest concentrations in the liver. ALDH1 and ALDH2 display different abilities to detoxify small aldehydes (such as acetaldehyde) and large
aldehydes (such as retinaldehyde). The assay solution uses acetaldehyde as a substrate, and thus preferentially detects the activity of ALDH2 in
crude extracts. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction.
The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the
presence of increased ALDH activity. The enzyme assay solution should be kept at -80ºC, and will remain stable at -80ºC for ~1 year. The product
is for research use only.
Kit Components:
Hexokinase Assay Solution: 10 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: acetaldehyde, TX-100, DMSO, INT, acetic acid, Hepes
Related kits: ADH Enzyme Assay, Ethanol Assay
Product Description: In mammals, ethanol is metabolized mainly in the liver by alcohol dehydrogenase (ADH), which oxidizes ethanol to
acetaldehyde. Acetaldehyde, a toxic metabolite responsible for the miserable effects of hangovers, is further oxidized to acetate by aldehyde
dehydrogenase (ALDH). ALDH belongs to a large family of aldehyde dehydrogenases that can be found in many tissues of the body, but are at
the highest concentrations in the liver. ALDH1 and ALDH2 display different abilities to detoxify small aldehydes (such as acetaldehyde) and large
aldehydes (such as retinaldehyde). The assay solution uses acetaldehyde as a substrate, and thus preferentially detects the activity of ALDH2 in
crude extracts. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction.
The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the
presence of increased ALDH activity. The enzyme assay solution should be kept at -80ºC, and will remain stable at -80ºC for ~1 year. The product
is for research use only.
Kit Components:
Hexokinase Assay Solution: 10 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: acetaldehyde, TX-100, DMSO, INT, acetic acid, Hepes
Related kits: ADH Enzyme Assay, Ethanol Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Hamster heart and liver extracts were
prepared, and assayed by ALDH
Assay Kit. Enzyme activities were
expressed as units per mg proteins
using a purified ALDH as standard.
The study shows that the liver exhibits
a more than 30 fold higher ALDH
activity than the heart. Enzyme units
per mg proteins are shown.
prepared, and assayed by ALDH
Assay Kit. Enzyme activities were
expressed as units per mg proteins
using a purified ALDH as standard.
The study shows that the liver exhibits
a more than 30 fold higher ALDH
activity than the heart. Enzyme units
per mg proteins are shown.