Alkaline Phosphatase (ALP) Assay Kit                                                                                                                                                              Price

Product Description: Alkaline phosphatase (ALP or ALK) possesses a hydrolytic activity removing phosphate groups from protein and non-protein
molecules. ALP is present in all mammalian tissues, and multiple isozymes are differentially distributed in the body. While abnormal ALP levels can
signal a wide range of clinical conditions, the primary importance of measuring ALP is diagnosis of bone or liver disease, although an elevated ALP
usually requires other tests to determine the origin of the pathology. ALP elevation also serves as an osteogenic differentiation marker. The enzyme
assay kit is based on ALP-mediated conversion of p-nitrophenol phosphate (PNPP) to nitrophenol in an alkaline buffer. The product nitrophenol
exhibits an absorption maximum at 405-415 nm, allowing for sensitive detection of ALP activity in crude cell/tissue extracts and serum/plasma. The
assay is designed for the 96-well plate format, but can be scaled up if desired. Repeated freeze-thaw cycles of the assay solution should be avoided.

Kit Components:

10x Cell Lysis Solution: 25 ml, store at 4ºC
ALP Assay Solution: 40 ml, store in aliquots at -20ºC (400 assays)

MSDS: TX-100, nitrophenol phosphate, Tris, NaOH

Related kits: beta-Galactosidase (LacZ) Assay, beta-Galactosidase (LacZ) Staining, Luciferase Assay, Osteogenesis Assay, Chondrogenesis Assay

Citation:
Leiker et al
Assessment of a nuclear affinity labeling method for tracking implanted mesenchymal stem cells
Cell Transplantation 17:911-922, 2008

J Cell Physiology 216:458, 2008
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Biomedical Research Service
& Clinical Application
Early and late passage bone marrow
mesenchymal stem cells were solubilized
in a lysis buffer, and cell lysates were
mixed with ALP (Alk) and Gal assay
solutions. The assays demonstrate
differential changes in enzyme activities
associated with adult stem cell senescence
in culture.
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