Alkaline Phosphatase Assay Kit Price
Product Description: Alkaline phosphatase (ALP or ALK) possesses a hydrolytic activity removing phosphate groups from protein and non-protein
molecules. ALP is present in all mammalian tissues, and multiple isozymes are differentially distributed in the body. While abnormal ALP levels can
signal a wide range of clinical conditions, the primary importance of measuring ALP is diagnosis of bone or liver disease, although an elevated ALP
usually requires other tests to determine the origin of the pathology. ALP elevation also serves as an osteogenic differentiation marker. The enzyme
assay kit is based on ALP-mediated conversion of p-nitrophenol phosphate (PNPP) to nitrophenol in an alkaline buffer. The product nitrophenol
exhibits an absorption maximum at 405-415 nm, allowing for sensitive detection of ALP activity in crude cell/tissue extracts and serum/plasma. The
assay is designed for the 96-well plate format, but can be scaled up if desired. Repeated freeze-thaw cycles of the assay solution should be avoided.
The product is for research use only.
Kit Components:
10x Cell Lysis Solution: 25 ml, store at RT
ALP Assay Solution: 50 ml, store in aliquots at -20ºC
MSDS: TX-100, nitrophenol phosphate, Tris, NaOH
Related kits: beta-Galactosidase (LacZ) Assay, beta-Galactosidase (LacZ) Staining, Luciferase Assay, Osteogenesis Assay, Chondrogenesis Assay
Product Description: Alkaline phosphatase (ALP or ALK) possesses a hydrolytic activity removing phosphate groups from protein and non-protein
molecules. ALP is present in all mammalian tissues, and multiple isozymes are differentially distributed in the body. While abnormal ALP levels can
signal a wide range of clinical conditions, the primary importance of measuring ALP is diagnosis of bone or liver disease, although an elevated ALP
usually requires other tests to determine the origin of the pathology. ALP elevation also serves as an osteogenic differentiation marker. The enzyme
assay kit is based on ALP-mediated conversion of p-nitrophenol phosphate (PNPP) to nitrophenol in an alkaline buffer. The product nitrophenol
exhibits an absorption maximum at 405-415 nm, allowing for sensitive detection of ALP activity in crude cell/tissue extracts and serum/plasma. The
assay is designed for the 96-well plate format, but can be scaled up if desired. Repeated freeze-thaw cycles of the assay solution should be avoided.
The product is for research use only.
Kit Components:
10x Cell Lysis Solution: 25 ml, store at RT
ALP Assay Solution: 50 ml, store in aliquots at -20ºC
MSDS: TX-100, nitrophenol phosphate, Tris, NaOH
Related kits: beta-Galactosidase (LacZ) Assay, beta-Galactosidase (LacZ) Staining, Luciferase Assay, Osteogenesis Assay, Chondrogenesis Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Measuring the activities of alkaline
phosphatase (Alk) and beta-galactosidase
(Gal) in crude cell extracts. Early and late
passage bone marrow mesenchymal
stem cells were solubilized in a lysis
buffer, and cell lysates were mixed with
Alk and Gal assay solutions. The assays
demonstrate differential changes in
enzyme activities associated with adult
stem cell senescence in culture.
phosphatase (Alk) and beta-galactosidase
(Gal) in crude cell extracts. Early and late
passage bone marrow mesenchymal
stem cells were solubilized in a lysis
buffer, and cell lysates were mixed with
Alk and Gal assay solutions. The assays
demonstrate differential changes in
enzyme activities associated with adult
stem cell senescence in culture.