Aspartate Aminotransferase (AST) Assay Kit Price
Product Description: Aspartate aminotransferase (AST) is a member of the transaminase family of enzymes. The enzyme is previously referred
to as glutamate oxaloacetate transaminase (GOT), which indicates its biological function in intermediary metabolism of glucose and protein
through catalyzing the transfer of an amino group between aspartate and glutamate. AST is found in many tissues throughout the body, including
the liver, heart, muscle, brain, and kidney, and therefore AST measurement along with ALT measurement is routinely performed in clinical
diagnostics. While serum levels of AST are elevated in most types of liver disease, increased serum AST may also be associated with myocardial
infarction, renal damage, and muscle necrosis. Assessment of the ratio of AST to ALT also provides clinically useful information. The colorimetric
AST assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and
exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased AST activity. The kit is
stable for at least one year if handled and stored properly. The product is for research use only.
Kit Components:
AST Assay Solution: 10 ml, store at -80°C (for 200 assays)
AST Substrate: 0.5 ml, store at -80°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: ALT Assay, HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay,
G6PD Enzyme Assay
Product Description: Aspartate aminotransferase (AST) is a member of the transaminase family of enzymes. The enzyme is previously referred
to as glutamate oxaloacetate transaminase (GOT), which indicates its biological function in intermediary metabolism of glucose and protein
through catalyzing the transfer of an amino group between aspartate and glutamate. AST is found in many tissues throughout the body, including
the liver, heart, muscle, brain, and kidney, and therefore AST measurement along with ALT measurement is routinely performed in clinical
diagnostics. While serum levels of AST are elevated in most types of liver disease, increased serum AST may also be associated with myocardial
infarction, renal damage, and muscle necrosis. Assessment of the ratio of AST to ALT also provides clinically useful information. The colorimetric
AST assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and
exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased AST activity. The kit is
stable for at least one year if handled and stored properly. The product is for research use only.
Kit Components:
AST Assay Solution: 10 ml, store at -80°C (for 200 assays)
AST Substrate: 0.5 ml, store at -80°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: ALT Assay, HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay,
G6PD Enzyme Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Plasma samples from 3-month and
30-month old Balb/c mice were
assayed with the AST Assay kit.
Enzyme activities were expressed
as O.D. units. This assay shows an
increased circulating level of AST
in the aging mice.
30-month old Balb/c mice were
assayed with the AST Assay kit.
Enzyme activities were expressed
as O.D. units. This assay shows an
increased circulating level of AST
in the aging mice.