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ATP Assay Kit

Product Description: Intracellular ATP concentrations vary during cell growth and cell death.
Notably, cells maintain a critical threshold of ATP levels, which dictates whether apoptotic or
necrotic cell death may take place in conditions of stress. Evaluation of cellular ATP levels
may thus provide useful information regarding the metabolic state and integrity of the cell. The
enzyme luciferase catalyzes, in the presence of ATP (see Luciferase Assay Kit), the oxidation
of luciferin with concomitant emission of yellow-green light, which can be conveniently
measured by scintillation counters or luminometers. Light emission peaks in several seconds
at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid
appearance and decay of the light flash requires consistent timing of the light measurement to
obtain reliable data. This provides the basis for a sensitive non-radioactive ATP assay system
more convenient than that based on HPLC. The kit is sufficient for 200 assays using 0.1 ml of
ATP Assay Solution per assay. The ATP Assay Solution if stored in aliquots at -75°C is stable
for at least one year from the time of purchase. Repeated freeze-thaw cycles should be
avoided. The kit does not include reagents required for extracting ATP from cells or tissues.
The product is for research use only.

Biomedical Research Service