ATP Assay Kit Price
Product Description: Intracellular ATP concentrations vary during cell growth and cell death. Notably, cells maintain a critical threshold of ATP
levels, which dictates whether apoptotic or necrotic cell death may take place in conditions of stress. Evaluation of cellular ATP levels may thus
provide useful information regarding the metabolic state and integrity of the cell. The enzyme luciferase catalyzes, in the presence of ATP, the
oxidation of luciferin with concomitant emission of yellow-green light, which can be conveniently measured by a luminometer or scintillation counter.
Light emission peaks in several seconds at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance
and decay of the light flash requires consistent timing of the light measurement to obtain reliable data. This provides the basis for a sensitive
non-radioactive ATP assay system capable of detecting 0.1 uM or 1 picomole of ATP and more convenient than that based on HPLC. The kit is
sufficient for 200 assays using 0.1 ml of ATP Assay Solution per assay. The ATP Assay Solution if stored in aliquots at -80°C is stable for many
years. Repeated freeze-thaw cycles should be avoided. The product is for research use only.
Kit Components:
ATP Assay Solution: 20 ml, store at -80°C (for 200 assays)
1 mM ATP Standard: 0.5 ml, store at -80°C
MSDS: luciferin, EDTA, Tris, DTT
Related kits: Cell Proliferation Assay, Cell Injury Assay, Phosphate Assay, NAD+ Assay, Pyruvate Assay, NAD+ Assay
Product Description: Intracellular ATP concentrations vary during cell growth and cell death. Notably, cells maintain a critical threshold of ATP
levels, which dictates whether apoptotic or necrotic cell death may take place in conditions of stress. Evaluation of cellular ATP levels may thus
provide useful information regarding the metabolic state and integrity of the cell. The enzyme luciferase catalyzes, in the presence of ATP, the
oxidation of luciferin with concomitant emission of yellow-green light, which can be conveniently measured by a luminometer or scintillation counter.
Light emission peaks in several seconds at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance
and decay of the light flash requires consistent timing of the light measurement to obtain reliable data. This provides the basis for a sensitive
non-radioactive ATP assay system capable of detecting 0.1 uM or 1 picomole of ATP and more convenient than that based on HPLC. The kit is
sufficient for 200 assays using 0.1 ml of ATP Assay Solution per assay. The ATP Assay Solution if stored in aliquots at -80°C is stable for many
years. Repeated freeze-thaw cycles should be avoided. The product is for research use only.
Kit Components:
ATP Assay Solution: 20 ml, store at -80°C (for 200 assays)
1 mM ATP Standard: 0.5 ml, store at -80°C
MSDS: luciferin, EDTA, Tris, DTT
Related kits: Cell Proliferation Assay, Cell Injury Assay, Phosphate Assay, NAD+ Assay, Pyruvate Assay, NAD+ Assay
 | |  | |  | |  | |  | |  | |  | |  | |  | |  | |  |
Biomedical Research Service
& Clinical Diagnostics
& Clinical Diagnostics
Porcine hibernating myocardium
received intra-coronary infusion of bone
marrow mesenchymal stem cells. Heart
tissues were harvested after one month
for ATP assays. The study shows that the
stem cell therapy improves cardiac
bioenergetics in the hibernating
myocardium
received intra-coronary infusion of bone
marrow mesenchymal stem cells. Heart
tissues were harvested after one month
for ATP assays. The study shows that the
stem cell therapy improves cardiac
bioenergetics in the hibernating
myocardium