ATP Assay Kit
Product Description: Intracellular ATP concentrations vary during cell growth and cell death. Notably, cells maintain a critical threshold of ATP
levels, which dictates whether apoptotic or necrotic cell death may take place in conditions of stress. Evaluation of cellular ATP levels may thus
provide useful information regarding the metabolic state and integrity of the cell. The enzyme luciferase catalyzes, in the presence of ATP, the
oxidation of luciferin with concomitant emission of yellow-green light, which can be conveniently measured by scintillation counters or luminometers.
Light emission peaks in several seconds at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance
and decay of the light flash requires consistent timing of the light measurement to obtain reliable data. This provides the basis for a sensitive
non-radioactive ATP assay system capable of detecting 0.1 uM or 1 picomole of ATP and more convenient than that based on HPLC. The kit is
sufficient for 200 assays using 0.1 ml of ATP Assay Solution per assay. The ATP Assay Solution if stored in aliquots at -80°C is stable for at least
one year from the time of purchase. Repeated freeze-thaw cycles should be avoided. The kit does not include reagents required for extracting ATP
from cells or tissues. The product is for research use only.
Kit Components:
ATP Assay Buffer: 20 ml, store at 4°C (for 200 assays)
ATP Assay Substrate: 1 vial (contents to be dissolved in 20 ml ATP Assay Buffer), store at -80°C
Luciferase Stock Solution: 25 ul, store at -80°C
1 mM ATP Standard: 0.5 ml, store at -80°C
MSDS: luciferin, EDTA, Tris, DTT
Related kits: Cell Proliferation Assay, Cell Injury Assay, Phosphate Assay
Product Description: Intracellular ATP concentrations vary during cell growth and cell death. Notably, cells maintain a critical threshold of ATP
levels, which dictates whether apoptotic or necrotic cell death may take place in conditions of stress. Evaluation of cellular ATP levels may thus
provide useful information regarding the metabolic state and integrity of the cell. The enzyme luciferase catalyzes, in the presence of ATP, the
oxidation of luciferin with concomitant emission of yellow-green light, which can be conveniently measured by scintillation counters or luminometers.
Light emission peaks in several seconds at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance
and decay of the light flash requires consistent timing of the light measurement to obtain reliable data. This provides the basis for a sensitive
non-radioactive ATP assay system capable of detecting 0.1 uM or 1 picomole of ATP and more convenient than that based on HPLC. The kit is
sufficient for 200 assays using 0.1 ml of ATP Assay Solution per assay. The ATP Assay Solution if stored in aliquots at -80°C is stable for at least
one year from the time of purchase. Repeated freeze-thaw cycles should be avoided. The kit does not include reagents required for extracting ATP
from cells or tissues. The product is for research use only.
Kit Components:
ATP Assay Buffer: 20 ml, store at 4°C (for 200 assays)
ATP Assay Substrate: 1 vial (contents to be dissolved in 20 ml ATP Assay Buffer), store at -80°C
Luciferase Stock Solution: 25 ul, store at -80°C
1 mM ATP Standard: 0.5 ml, store at -80°C
MSDS: luciferin, EDTA, Tris, DTT
Related kits: Cell Proliferation Assay, Cell Injury Assay, Phosphate Assay
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Biomedical Research Service
& Clinical Diagnostics
& Clinical Diagnostics
Porcine hibernating myocardium
received intra-coronary infusion of bone
marrow mesenchymal stem cells. Heart
tissues were harvested after one month
for ATP assays. The study shows that the
stem cell therapy improves cardiac
bioenergetics in the hibernating
myocardium
received intra-coronary infusion of bone
marrow mesenchymal stem cells. Heart
tissues were harvested after one month
for ATP assays. The study shows that the
stem cell therapy improves cardiac
bioenergetics in the hibernating
myocardium