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Adipogenesis Assay Kit

Product Description: Adipogenesis is associated with upregulation of many adipocyte-specific
genes, culminating in the synthesis of fatty acids which are stored in the cytosol as
triacylglycerols. These lipid molecules are water insoluble and coalesce to form fat globules
visible to the naked eyes. The Oil Red dye is hydrophobic displaying a distinct deep red color.
This dye is commonly used in staining protocols to demonstrate the presence of intracellular
lipids and lipid-bound proteins. Since the synthesis of lipids is increased during adipogenesis,
staining with Oil Red is a fast and easy method to identify cells undergoing adipogenesis. The
lipid-bound Oil Red is readily identifiable by microscopy, and can be extracted with an organic
solvent after staining and washing, which then allows one to assay adipogenesis quantitatively
by spectroscopy. The assay can be set up in a microplate format.

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