Protein Assay Kit/DC (detergent compatible) Price
Product Description: The detergent compatible Protein Assay Kit combines two famous reactions- the Biuret reaction and the Smith reaction. The
Biuret reaction depicts the reduction of cupric ion (Cu2+) to cuprous ion (Cu1+) by protein in an alkaline solution. The intensity of the reaction is
proportional to the number of peptide bonds (at least 2 peptide bonds) and therefore to the number of protein molecules present in the sample
solution. The Smith reaction depicts the chelation of each Cu1+ ion by two molecules of bicinchoninic acid (BCA), resulting in an intense purple-
colored product that absorbs light at 562 nm (Anal. Biochem. 150: 76–85, 1985), which can be measured with a standard laboratory
spectrophotometer or microplate reader. Unlike the dye-based protein assay system, the BCA-based protein assay system is compatible with
detergent-containing protein samples. However, the BCA assay is incompatible with most divalent ions. Prepare fresh working solution by mixing 50
parts of BCA Solution with 1 part of Copper Solution (50:1). The Protein Assay System has a broad working range of 20 - 2000 ug/ml proteins. The
assay kit also includes 1 ml of 100 mg/ml sterilized BSA dissolved in distilled water to be diluted for use as protein standard. The assay kit is stable
for at least one year under proper storage and handling conditions. The product is for research use only.
Kit Components:
BCA Solution: 200 ml, store at room temperature
Copper Solution: 5 ml, store at room temperature
100 mg/ml Bovine Serum Albumin (BSA): 1 ml, store at -20ºC
MSDS: NaOH, BCA, Potassium sodium tartrate, Cupric sulfate
Related kits: Protein Assay Kit (EDTA/Mg+2 compatible), Enhanced Chemiluminescence (ECL) Detection (Luminol substrate), ELISA detection
(TMB substrate)
Product Description: The detergent compatible Protein Assay Kit combines two famous reactions- the Biuret reaction and the Smith reaction. The
Biuret reaction depicts the reduction of cupric ion (Cu2+) to cuprous ion (Cu1+) by protein in an alkaline solution. The intensity of the reaction is
proportional to the number of peptide bonds (at least 2 peptide bonds) and therefore to the number of protein molecules present in the sample
solution. The Smith reaction depicts the chelation of each Cu1+ ion by two molecules of bicinchoninic acid (BCA), resulting in an intense purple-
colored product that absorbs light at 562 nm (Anal. Biochem. 150: 76–85, 1985), which can be measured with a standard laboratory
spectrophotometer or microplate reader. Unlike the dye-based protein assay system, the BCA-based protein assay system is compatible with
detergent-containing protein samples. However, the BCA assay is incompatible with most divalent ions. Prepare fresh working solution by mixing 50
parts of BCA Solution with 1 part of Copper Solution (50:1). The Protein Assay System has a broad working range of 20 - 2000 ug/ml proteins. The
assay kit also includes 1 ml of 100 mg/ml sterilized BSA dissolved in distilled water to be diluted for use as protein standard. The assay kit is stable
for at least one year under proper storage and handling conditions. The product is for research use only.
Kit Components:
BCA Solution: 200 ml, store at room temperature
Copper Solution: 5 ml, store at room temperature
100 mg/ml Bovine Serum Albumin (BSA): 1 ml, store at -20ºC
MSDS: NaOH, BCA, Potassium sodium tartrate, Cupric sulfate
Related kits: Protein Assay Kit (EDTA/Mg+2 compatible), Enhanced Chemiluminescence (ECL) Detection (Luminol substrate), ELISA detection
(TMB substrate)
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Biomedical Research Service
& Clinical Application
& Clinical Application
The Protein Assay System has a linear working range of
20 - 2000 ug/ml proteins. The assay is compatible with
detergents. Use the Protein Assay Kit 595 nm System
for samples containing reducing agents (DTT), chelating
agents (EDTA), and divalent cations.
20 - 2000 ug/ml proteins. The assay is compatible with
detergents. Use the Protein Assay Kit 595 nm System
for samples containing reducing agents (DTT), chelating
agents (EDTA), and divalent cations.