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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Catalase Assay Kit
Product Description: Catalase, present in nearly all aerobic cells, serves to protect the cell
against oxidative damage caused by hydrogen peroxide (H2O2). Hydrogen peroxide, although
not a free radical, is highly reactive. Catalase catalyzes its decomposition into molecular
oxygen and water without the production of free radicals. Catalase also uses hydrogen
peroxide to oxidize toxins including phenols, formic acid, formaldehyde, and alcohols. The
beef liver catalase monomer consists of 506 amino acids, a heme prosthetic group, and one
NADH molecule. The enzyme is among the most efficient known, with rates approaching
200,000 catalytic events/second/subunit, which is near the diffusion-controlled limit. Our
catalase assay system is based on the ability of hydrogen peroxide to oxidize an azo
chromogen with an accompanying increase in O.D.410-420 nm, which can be conveniently
quantified by spectroscopy. The presence of catalase reduces the amount of hydrogen
peroxide, thus inhibiting the oxidation of the azo chromogen and the appearance of the
yellowish color. This inhibition of chromogen oxidation is thus proportional to the activity of
catalase present in the sample. As little as 103-104 cells can be used per assay. The assay is
simple, non-radioactive, and very sensitive. Each kit is sufficient for 250 microassays.
Biomedical Research Service
