Catalase Assay Kit Price
Product Description: Catalase, present in nearly all aerobic cells, serves to protect the cell against oxidative damage caused by hydrogen
peroxide (H2O2). Hydrogen peroxide, although not a free radical, is highly reactive. Catalase catalyzes its decomposition into molecular oxygen
and water without the production of free radicals. Catalase also uses hydrogen peroxide to oxidize toxins including phenols, formic acid,
formaldehyde, and alcohols. The beef liver catalase monomer consists of 506 amino acids, a heme prosthetic group, and one NADH molecule.
The enzyme is among the most efficient known, with rates approaching 200,000 catalytic events/second/subunit, which is near the
diffusion-controlled limit. Our catalase assay system is based on the ability of hydrogen peroxide to oxidize an azo chromogen with an
accompanying increase in O.D.410-420 nm, which can be conveniently quantified by spectroscopy. The presence of catalase reduces the amount
of hydrogen peroxide, thus inhibiting the oxidation of the azo chromogen and the appearance of the yellowish color. This inhibition of chromogen
oxidation is thus proportional to the activity of catalase present in the sample. As little as 103-104 cells can be used per assay. The assay is
simple, non-radioactive, and very sensitive. Each kit is sufficient for 250 microassays.
Kit Components:
Catalase Substrate Solution: 25 ml, store at 4ºC
Catalase Assay Solution: 25 ml, store at 4ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: H2O2, TX-100
Related kits: Lipid Peroxidation Assay Kit, Peroxide Assay Kit
Product Description: Catalase, present in nearly all aerobic cells, serves to protect the cell against oxidative damage caused by hydrogen
peroxide (H2O2). Hydrogen peroxide, although not a free radical, is highly reactive. Catalase catalyzes its decomposition into molecular oxygen
and water without the production of free radicals. Catalase also uses hydrogen peroxide to oxidize toxins including phenols, formic acid,
formaldehyde, and alcohols. The beef liver catalase monomer consists of 506 amino acids, a heme prosthetic group, and one NADH molecule.
The enzyme is among the most efficient known, with rates approaching 200,000 catalytic events/second/subunit, which is near the
diffusion-controlled limit. Our catalase assay system is based on the ability of hydrogen peroxide to oxidize an azo chromogen with an
accompanying increase in O.D.410-420 nm, which can be conveniently quantified by spectroscopy. The presence of catalase reduces the amount
of hydrogen peroxide, thus inhibiting the oxidation of the azo chromogen and the appearance of the yellowish color. This inhibition of chromogen
oxidation is thus proportional to the activity of catalase present in the sample. As little as 103-104 cells can be used per assay. The assay is
simple, non-radioactive, and very sensitive. Each kit is sufficient for 250 microassays.
Kit Components:
Catalase Substrate Solution: 25 ml, store at 4ºC
Catalase Assay Solution: 25 ml, store at 4ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: H2O2, TX-100
Related kits: Lipid Peroxidation Assay Kit, Peroxide Assay Kit
 | |  | |  | |  | |  | |  | |  | |  | |  | |  | |  |
Biomedical Research Service
& Clinical Application
& Clinical Application
Normal hamster muscle exhibits a higher
level of catalase activity than dystrophic
hamster muscle (MD) as determined by the
Catalase Assay Kit. After mesenchymal
stem cell therapy for the muscular
dystrophy, the stem cell-injected muscle
displays a normalized level of catalase
activity.
level of catalase activity than dystrophic
hamster muscle (MD) as determined by the
Catalase Assay Kit. After mesenchymal
stem cell therapy for the muscular
dystrophy, the stem cell-injected muscle
displays a normalized level of catalase
activity.