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Cell Injury Assay Kit

Product Description: The kinetic determination of the cellular enzyme lactate dehydrogenase
(LDH) released upon cell damage or lysis is a sensitive, precise, and convenient method for
the studies of apoptosis and cytotoxicity reactions (J. Immunol. Methods 64:313,1983). The
inexpensive, non-radioactive colorimetric LDH assay provides a superb alternative to 51Cr
release assays (Immunology 14:181,1968), which often suffer from higher background levels.
The Cell Injury Assay Kit is based on the reduction of the tetrazolium salt INT in a
NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an
absorption maximum at 492 nm. Since the intensity of the red color formed is proportional to
the number of damaged or lysed cells, the assay has been widely used for the detection and
quantification of apoptosis as well as necrosis (Exp. Cell Res. 226:372, 1996) (see Cell
Viability/Proliferation Assay Kit). The superior sensitivity of the non-radioactive assay kit allows
the assay to be completed within 30 min with minimal pipetting steps. Each kit is sufficient for
approximately 300 assays using the 96-well format. See related product Lactate Assay Kit.
The method is adaptable to automation.

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