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Chondrogenesis Assay Kit

Product Description: Alcian blue, a group of polyvalent basic dyes that are water soluble,
stains acid mucosubstances (sulfated and carboxylated mucopolysaccharides) and
sialomucins (glycoproteins), both of which are cartilaginous elements. Since the synthesis of
these extracellular macromolecules is increased during chondrogensis, staining with Alcian
blue is a fast and easy method to identify cells undergoing chondrogenesis. Cell-bound Alcian
blue can be extracted with an organic solvent after staining and washing, which then allows
one to assay chondrogenesis quantitatively by spectroscopy. The assay can be set up in a
microplate format.

Biomedical Research Service