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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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DNA Footprinting Assay Kit
Product Description: DNAase I in the presence of Mg2+ hydrolyzes each strand of DNA
independently in a statistically random fashion. This property of the nuclease proves to be
quite useful in the analysis of DNA-protein complexes (in a technique commonly referred to
as DNA footprinting assay; Nucl. Acids Res. 5:3157,1978) since the protein-bound region is
often protected from the attack of properly diluted DNAase I. Information regarding structural
features of DNA such as the distribution of DNAase I hyper-sensitive sites within the DNA
segment of interest is also readily obtainable. The footprinting assay, which is best
complemented by gel shift assay, can be performed with purified protein factors or with a
crude cell extract in the presence of a non-specific polyanion competitor, usually
double-stranded poly(dI-dC). The amount of poly(dI-dC) used per reaction can be much
reduced if purified protein fractions are used for the analysis of DNA-protein complexes. The
product is stable for at least 1 year if stored and handled properly, and is for research use
only.
Biomedical Research Service
