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Plasmid DNA is purified from DH5a strain using our Maxi-Prep protocol, and
typically has an A260/A280 absorption ratio of ~2. Service fee for each
plasmid is $180 (50 ug DNA).
I. Promoter-less Reporter Vectors (Your favorite gene promoter can be
inserted into these plasmids for transient transfection assays)
p-lacZ beta-galactosidase reporter
p-luc firefly luciferase reporter
p-cat chloramphenicol acetyl transferase
II. Promoter-Reporter Vectors (Expression of each of the following
reporter enzymes is driven by constitutive eukaryotic and viral
promoters/enhancers such as pSV, pCMV, pRSV, pMSV, pActin, pTK)
pActin-lacZ beta-galactosidase reporter
pCMV-lacZ beta-galactosidase reporter
pRSV-lacZ beta-galactosidase reporter
pSV-lacZ beta-galactosidase reporter
pCMV-luc firefly luciferase reporter
pSV-luc firefly luciferase reporter
pTK-luc firefly luciferase reporter
pActin-cat CAT reporter
pSV-cat CAT reporter
pTK-cat CAT reporter
pCMV-EGFP green fluorescent protein reporter
III. Expression Vectors (Your favorite gene can be inserted into the
vector for over-expression studies)
pSV Simian Virus 40 promoter
pMSV Moloney sarcoma virus promoter
pCMV Cytomegalovirus promoter
IV. GAL4 Fusion Vectors (These vectors encode a truncated yeast GAL4
DNA-binding domain (1-147), which can be fused in-frame with your favorite
gene. The fusion protein can be used to study nuclear localization,
transcriptional activation domain, and protein-protein interaction in
eukaryotic cells. Three different restriction site sequences were
engineered downstream from GAL4 (DB1, DB2, DB3).
pGAL4-DB1 GAL4 fusion protein
pGAL4-DB2 GAL4 fusion protein
pGAL4-DB3 GAL4 fusion protein
These reporter vectors contain either cat or luc reporters driven by a
minimal TATA box or a minimal TATA box in conjunction with 5 copies of the
GAL4 binding sites (G5) positioned upstream of the TATA box. Isolated
promoter elements can be inserted upstream of the G5 site for
transcriptional analysis.
pTATA-luc luciferase reporter
pG5-TATA-luc luciferase reporter
pG5-TATA-cat cat reporter
V. Yeast Two-Hybrid Vectors (These yeast vectors are used for constructing
GAL4-fusion proteins in yeast two-hybrid transcriptional analysis)
pYDB fusion with GAL4 DNA-binding domain
pYAC fusion with GAL4 activation domain
Plasmid DNA is purified from DH5a strain using our Maxi-Prep protocol, and
typically has an A260/A280 absorption ratio of ~2. Service fee for each
plasmid is $180 (50 ug DNA).
I. Promoter-less Reporter Vectors (Your favorite gene promoter can be
inserted into these plasmids for transient transfection assays)
p-lacZ beta-galactosidase reporter
p-luc firefly luciferase reporter
p-cat chloramphenicol acetyl transferase
II. Promoter-Reporter Vectors (Expression of each of the following
reporter enzymes is driven by constitutive eukaryotic and viral
promoters/enhancers such as pSV, pCMV, pRSV, pMSV, pActin, pTK)
pActin-lacZ beta-galactosidase reporter
pCMV-lacZ beta-galactosidase reporter
pRSV-lacZ beta-galactosidase reporter
pSV-lacZ beta-galactosidase reporter
pCMV-luc firefly luciferase reporter
pSV-luc firefly luciferase reporter
pTK-luc firefly luciferase reporter
pActin-cat CAT reporter
pSV-cat CAT reporter
pTK-cat CAT reporter
pCMV-EGFP green fluorescent protein reporter
III. Expression Vectors (Your favorite gene can be inserted into the
vector for over-expression studies)
pSV Simian Virus 40 promoter
pMSV Moloney sarcoma virus promoter
pCMV Cytomegalovirus promoter
IV. GAL4 Fusion Vectors (These vectors encode a truncated yeast GAL4
DNA-binding domain (1-147), which can be fused in-frame with your favorite
gene. The fusion protein can be used to study nuclear localization,
transcriptional activation domain, and protein-protein interaction in
eukaryotic cells. Three different restriction site sequences were
engineered downstream from GAL4 (DB1, DB2, DB3).
pGAL4-DB1 GAL4 fusion protein
pGAL4-DB2 GAL4 fusion protein
pGAL4-DB3 GAL4 fusion protein
These reporter vectors contain either cat or luc reporters driven by a
minimal TATA box or a minimal TATA box in conjunction with 5 copies of the
GAL4 binding sites (G5) positioned upstream of the TATA box. Isolated
promoter elements can be inserted upstream of the G5 site for
transcriptional analysis.
pTATA-luc luciferase reporter
pG5-TATA-luc luciferase reporter
pG5-TATA-cat cat reporter
V. Yeast Two-Hybrid Vectors (These yeast vectors are used for constructing
GAL4-fusion proteins in yeast two-hybrid transcriptional analysis)
pYDB fusion with GAL4 DNA-binding domain
pYAC fusion with GAL4 activation domain
biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
Biomedical Research Service
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