Plasmid DNA is purified from DH5a strain using our Maxi-Prep protocol, and typically has an A260/A280 absorption ratio of ~2. Service fee for each plasmid is $160 (50 ug DNA). Please include a $15 S/H fee for domestic overnight express shipping. I. Promoter-less Reporter Vectors (Your favorite gene promoter can be inserted into these plasmids for transient transfection assays) p-lacZ       beta-galactosidase reporter         p-luc        firefly luciferase reporter         p-cat        chloramphenicol acetyl transferase II. Promoter-Reporter Vectors (Expression of each of the following reporter enzymes is driven by constitutive eukaryotic and viral promoters/enhancers such as pSV, pCMV, pRSV, pMSV, pActin, pTK) pActin-lacZ  beta-galactosidase reporter         pCMV-lacZ    beta-galactosidase reporter         pRSV-lacZ    beta-galactosidase reporter         pSV-lacZ     beta-galactosidase reporter         pCMV-luc     firefly luciferase reporter         pSV-luc      firefly luciferase reporter         pTK-luc      firefly luciferase reporter         pActin-cat   CAT reporter                        pSV-cat      CAT reporter                        pTK-cat      CAT reporter                        pCMV-EGFP    green fluorescent protein reporter III. Expression Vectors (Your favorite gene can be inserted into the vector for over-expression studies) pSV          Simian Virus 40 promoter           pMSV         Moloney sarcoma virus promoter      pCMV         Cytomegalovirus promoter            IV. GAL4 Fusion Vectors (These vectors encode a truncated yeast GAL4 DNA-binding domain (1-147), which can be fused in-frame with your favorite gene. The fusion protein can be used to study nuclear localization, transcriptional activation domain, and protein-protein interaction in eukaryotic cells. Three different restriction site sequences were engineered downstream from GAL4 (DB1, DB2, DB3). pGAL4-DB1    GAL4 fusion protein               pGAL4-DB2    GAL4 fusion protein               pGAL4-DB3    GAL4 fusion protein          These reporter vectors contain either cat or luc reporters driven by a minimal TATA box or a minimal TATA box in conjunction with 5 copies of the GAL4 binding sites (G5) positioned upstream of the TATA box. Isolated promoter elements can be inserted upstream of the G5 site for transcriptional analysis. pTATA-luc    luciferase reporter        pG5-TATA-luc luciferase reporter                pG5-TATA-cat cat reporter      V. Yeast Two-Hybrid Vectors (These yeast vectors are used for constructing GAL4-fusion proteins in yeast two-hybrid transcriptional analysis) pYDB         fusion with GAL4 DNA-binding domain pYAC         fusion with GAL4 activation domain

biomedical research biomedical research biomedical research biomedical research biomedical research biomedical research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell competent cell competent cell biomedical research service biomedical research service biomedical research

Biomedical Research Service