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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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DNA Transfection Kit
Product Description: The ability to introduce exogenous plasmid DNA into cells (DNA
transfection) has resulted in many advances in molecular and cellular biology. The most
frequently used DNA transfer methods are those based on the use of calcium phosphate,
DEAE dextran, liposome (lipofectin), and electroporation (BioTechniques 13:598,1992).
However, efficiency of DNA transfection and reagent toxicity vary widely between different cell
types and cell lines. Adequate gene expression in one cell system may therefore not predict a
similar situation in another cell system. Our DNA Transfection Kit (sufficient for 200
transfection assays) is based on a unique method incorporating DNA into calcium phosphate
nanoparticles in the absence (DNA Particle Solution-A) or presence (DNA Particle Solution-B)
of a cationic polymer. The two particle formulations allow you to maximize transfection
efficiency in different cell systems. This inexpensive, convenient transfection kit involves only
mixing your DNA and the transfection reagents, and requires no specialized equipment.
Components of the kit are stable for at least one year if handled and stored properly. The
DNA transfection Kit has been found to be far more superior to lipofectin in the transfection of
R28 cells. It has been found to work well for cell lines including but not limited to 293 cells,
Sol8 cells, bone marrow-derived mesenchymal stem cells, and P19 cells.
Biomedical Research Service
