biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
DNA Transfection Kit
PRODUCT DESCRIPTION: The most frequently used DNA transfer methods are those based
on the use of calcium phosphate, DEAE dextran, liposome, and electroporation
(BioTechniques 13:598,1992). However, efficiency of DNA transfection and reagent toxicity
vary widely between different cell types and cell lines. Our DNA Transfection Kit is based on
incorporation of DNA into uniquely formulated nanoparticles in the absence (Nano A) or
presence (Nano B) of a polymer. The two nanoparticle formulations allow you to optimize
transfection efficiency for different cell systems. The use of Nano A or Nano B should be
determined empirically for each cell type. For instance, transfection of C2C12 myocytes with
Nano A can yield a 10-fold higher transfection efficiency than with Nano B. On the other hand,
Sol8 myocytes can be more efficiently transfected with Nano B. The kit is stable for at least
one year if handled and stored properly.
PRODUCT DESCRIPTION: The most frequently used DNA transfer methods are those based
on the use of calcium phosphate, DEAE dextran, liposome, and electroporation
(BioTechniques 13:598,1992). However, efficiency of DNA transfection and reagent toxicity
vary widely between different cell types and cell lines. Our DNA Transfection Kit is based on
incorporation of DNA into uniquely formulated nanoparticles in the absence (Nano A) or
presence (Nano B) of a polymer. The two nanoparticle formulations allow you to optimize
transfection efficiency for different cell systems. The use of Nano A or Nano B should be
determined empirically for each cell type. For instance, transfection of C2C12 myocytes with
Nano A can yield a 10-fold higher transfection efficiency than with Nano B. On the other hand,
Sol8 myocytes can be more efficiently transfected with Nano B. The kit is stable for at least
one year if handled and stored properly.
Biomedical Research Service
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