E coli Competent Cell Preparation Kit Price
Product Description: Bacteria can take up and stably maintain exogenous plasmid DNA in a process referred to as genetic transformation. The
efficiency of transformation depends on a transitory state of host cell competence, which, although varies significantly among different bacterial
strains, can be greatly enhanced through chemical treatments or electroporation. For most cloning experiments, however, competent cells
capable of generating 10^6 - 10^7 cfu/ug plasmid DNA can be routinely used. Some but not all E. coli strains can be made highly competent using
our enhanced calcium chloride-based method. A transformation efficiency of up to 10^8 cfu/ug pBluescript plasmid DNA may be obtained with
DH5a competent cells prepared with the E coli Competent Cell Preparation Kit. The prepared competent cells, if stored at -75ºC in aliquots,
remain competent indefinitely. The product is for research use only.
Kit Components:
E. coli Wash Solution: 200 ml, store at 4°C
E. coli Treatment Solution: 200 ml, store at 4°C
E. coli Stabilization Solution: 20 ml, store at 4°C
MSDS: Tris
Related kits: Whole Blood DNA Isolation, Plasmid DNA Isolation, High-Range DNA Markers, Low-Range DNA Markers
Product Description: Bacteria can take up and stably maintain exogenous plasmid DNA in a process referred to as genetic transformation. The
efficiency of transformation depends on a transitory state of host cell competence, which, although varies significantly among different bacterial
strains, can be greatly enhanced through chemical treatments or electroporation. For most cloning experiments, however, competent cells
capable of generating 10^6 - 10^7 cfu/ug plasmid DNA can be routinely used. Some but not all E. coli strains can be made highly competent using
our enhanced calcium chloride-based method. A transformation efficiency of up to 10^8 cfu/ug pBluescript plasmid DNA may be obtained with
DH5a competent cells prepared with the E coli Competent Cell Preparation Kit. The prepared competent cells, if stored at -75ºC in aliquots,
remain competent indefinitely. The product is for research use only.
Kit Components:
E. coli Wash Solution: 200 ml, store at 4°C
E. coli Treatment Solution: 200 ml, store at 4°C
E. coli Stabilization Solution: 20 ml, store at 4°C
MSDS: Tris
Related kits: Whole Blood DNA Isolation, Plasmid DNA Isolation, High-Range DNA Markers, Low-Range DNA Markers
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Biomedical Research Service
& Clinical Application
& Clinical Application
E. coli DH5a competent cells were prepared using the
Competent Cell Preparation Kit. Cells were transformed
with pDrive cloning vector (Qiagen) hybridized to a Taq
polymerase-amplified cDNA insert. LacZ-based blue/white
selection was used to identify colonies containing the
cDNA insert. The agar plate containing Xgal and
kanamycin harbored both blue (negative) and white
(positive) colonies.
Competent Cell Preparation Kit. Cells were transformed
with pDrive cloning vector (Qiagen) hybridized to a Taq
polymerase-amplified cDNA insert. LacZ-based blue/white
selection was used to identify colonies containing the
cDNA insert. The agar plate containing Xgal and
kanamycin harbored both blue (negative) and white
(positive) colonies.