Ethanol Assay Kit Price
Product Description: Alcoholic drinks vary widely in ethanol content, and the concentration of alcohol is usually marked as the percentage of
alcohol by volume (ABV) or as proof (e.g., 100 proof = 50% ABV). Blood alcohol content (BAC) is most commonly used in medical or legal arenas.
Serious intoxication can be observed with a BAC reading of 0.2%, and 0.4% is the accepted LD50 for adult humans. Being a central nervous
system depressant, ethanol has profound psychoactive effects in sub-lethal doses. The assay kit takes advantage of the narrow specificity of
yeast alcohol dehydrogenase (ADH), and thus primarily detects ethanol, although other straight chain primary alcohols and aldehydes also react
with the assay solution. The kit is designed to measure ethanol in serum, urine, food beverages, and culture medium samples. Assay is based on
the reduction of INT in a NADH-coupled reaction to formazan, which exhibits an absorption maximum at 492 nm. The intensity of the red color
formed is proportional to sample ethanol concentrations with a linear range of 0.01 - 0.2% ethanol. Since the assay reaction is enzymatic in
nature, non-specific interference may occur with certain complex samples. The assay solution should be kept at -80ºC for longer-term storage
(more than 1 month). The product is for research use only.
Kit Components:
Ethanol Assay Solution: 10 ml, store at -20°C or -80°C
MSDS: DMSO, INT, acetic acid, Hepes
Related kits: ADH Assay, ALDH Assay
Product Description: Alcoholic drinks vary widely in ethanol content, and the concentration of alcohol is usually marked as the percentage of
alcohol by volume (ABV) or as proof (e.g., 100 proof = 50% ABV). Blood alcohol content (BAC) is most commonly used in medical or legal arenas.
Serious intoxication can be observed with a BAC reading of 0.2%, and 0.4% is the accepted LD50 for adult humans. Being a central nervous
system depressant, ethanol has profound psychoactive effects in sub-lethal doses. The assay kit takes advantage of the narrow specificity of
yeast alcohol dehydrogenase (ADH), and thus primarily detects ethanol, although other straight chain primary alcohols and aldehydes also react
with the assay solution. The kit is designed to measure ethanol in serum, urine, food beverages, and culture medium samples. Assay is based on
the reduction of INT in a NADH-coupled reaction to formazan, which exhibits an absorption maximum at 492 nm. The intensity of the red color
formed is proportional to sample ethanol concentrations with a linear range of 0.01 - 0.2% ethanol. Since the assay reaction is enzymatic in
nature, non-specific interference may occur with certain complex samples. The assay solution should be kept at -80ºC for longer-term storage
(more than 1 month). The product is for research use only.
Kit Components:
Ethanol Assay Solution: 10 ml, store at -20°C or -80°C
MSDS: DMSO, INT, acetic acid, Hepes
Related kits: ADH Assay, ALDH Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Measuring ethanol content in Red Beaujolais Wine
using our Ethanol Assay kit. The Ethanol Assay
Solution was mixed with 50 ul of 200-fold diluted Red
Wine, and the mixture was incubated for 30 min.
O.D.492 nm was measured, and ethanol
concentration deduced from an ethanol standard
plot. The assay shows that the Red Wine contains
~13% alcohol.
using our Ethanol Assay kit. The Ethanol Assay
Solution was mixed with 50 ul of 200-fold diluted Red
Wine, and the mixture was incubated for 30 min.
O.D.492 nm was measured, and ethanol
concentration deduced from an ethanol standard
plot. The assay shows that the Red Wine contains
~13% alcohol.