Glucose-6-phosphate Dehydrogenase (G6PD) Assay Kit                                                                                                                             Price

Product Description: Glucose-6-phosphate dehydrogenase (G6PD), a soluble enzyme, catalyzes the rate-limiting and regulated step in the
pentose phosphate pathway, oxidizing glucose-6-phosphate with the production of NADPH, which is required for reductive biosynthesis and cellular
defense against microbial pathogens and reactive oxygen species. The enzyme is particularly important for maintaining the integrity of red blood
cells, and thus defective G6PD can cause hemolytic anemia in the presence of several precipitating factors (favism and infection). This
non-radioactive colorimetric G6PD assay is based on the reduction of the tetrazolium salt INT in a NADPH-coupled enzymatic reaction. The INT
reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence
of increased G6PD activity. The assay solution should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable
indefinitely at -80ºC.  

Kit Components:

G6PD Assay Solution: 10 ml, store at -20ºC or -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay, Glucose
Assay
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Biomedical Research Service
& Clinical Application
G6PD assays were performed using heart
extracts prepared from normal hamster
(F1B strain) and cardiomyopathic hamster
(TO2 strain). Enzyme activities were
expressed as units per mg proteins using a
purified G6PD as standard. The study shows
that the cardiomyopathic heart exhibits an
increased G6PD enzyme activity due to
sustained activation of the endogenous
cardiac repair mechanism. Enzyme units
per mg proteins are shown.
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