Glucose-6-phosphate Dehydrogenase (G6PD) Assay Kit Price
Product Description: Glucose-6-phosphate dehydrogenase (G6PD), a soluble enzyme, catalyzes the rate-limiting and regulated step in the
pentose phosphate pathway, oxidizing glucose-6-phosphate with the production of NADPH, which is required for reductive biosynthesis and cellular
defense against microbial pathogens and reactive oxygen species. The enzyme is particularly important for maintaining the integrity of red blood
cells, and thus defective G6PD can cause hemolytic anemia in the presence of several precipitating factors (favism and infection). This
non-radioactive colorimetric G6PD assay is based on the reduction of the tetrazolium salt INT in a NADPH-coupled enzymatic reaction. The INT
reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence
of increased G6PD activity. The assay solution should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable
indefinitely at -80ºC. The product is for research use only.
Kit Components:
G6PD Assay Solution: 10 ml, store at -20ºC or -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay, Glucose
Assay
Product Description: Glucose-6-phosphate dehydrogenase (G6PD), a soluble enzyme, catalyzes the rate-limiting and regulated step in the
pentose phosphate pathway, oxidizing glucose-6-phosphate with the production of NADPH, which is required for reductive biosynthesis and cellular
defense against microbial pathogens and reactive oxygen species. The enzyme is particularly important for maintaining the integrity of red blood
cells, and thus defective G6PD can cause hemolytic anemia in the presence of several precipitating factors (favism and infection). This
non-radioactive colorimetric G6PD assay is based on the reduction of the tetrazolium salt INT in a NADPH-coupled enzymatic reaction. The INT
reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence
of increased G6PD activity. The assay solution should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable
indefinitely at -80ºC. The product is for research use only.
Kit Components:
G6PD Assay Solution: 10 ml, store at -20ºC or -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay, Glucose
Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Heart tissue extracts were prepared
from normal hamster (F1B strain) and
cardiomyopathic hamster (TO2 strain),
and assayed by G6PD Assay Kit.
Enzyme activities were expressed as
units per mg proteins using a purified
G6PD as standard. The study shows
that the cardiomyopathic heart
exhibits an increased G6PD enzyme
activity due to sustained activation of
the endogenous cardiac repair
mechanism. Enzyme units per mg
proteins are shown.
from normal hamster (F1B strain) and
cardiomyopathic hamster (TO2 strain),
and assayed by G6PD Assay Kit.
Enzyme activities were expressed as
units per mg proteins using a purified
G6PD as standard. The study shows
that the cardiomyopathic heart
exhibits an increased G6PD enzyme
activity due to sustained activation of
the endogenous cardiac repair
mechanism. Enzyme units per mg
proteins are shown.