Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit Price
Product Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme mediating the conversion of
glyceraldehyde-3-phosphate to 1,3-bisphosphate glycerate with the production of NADH. Although this house-keeping gene is often used as
internal control for RNA and protein analysis, expression of the gene can be altered under certain cell growth and differentiation conditions. In
addition, a switch from aerobic metabolism to anaerobic glycolysis may also be accompanied by a change in GAPDH expression. The GAPDH
enzyme activity assay kit can be used to quickly assess potential changes in the expression of the gene. The non-radioactive colorimetric
GAPDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble
and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased GAPDH activity.
The kit is stable for at least one year if handled and stored properly.
Kit Components:
GAPDH Assay Solution: 5 ml, store at -20°C or -80°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, Glucose Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay,
G6PD Enzyme Assay
Citation:
Giulivi et al
Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia-susceptible R163C knock-in mice
J Biol Chem, 286:99-113, 2011
Product Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme mediating the conversion of
glyceraldehyde-3-phosphate to 1,3-bisphosphate glycerate with the production of NADH. Although this house-keeping gene is often used as
internal control for RNA and protein analysis, expression of the gene can be altered under certain cell growth and differentiation conditions. In
addition, a switch from aerobic metabolism to anaerobic glycolysis may also be accompanied by a change in GAPDH expression. The GAPDH
enzyme activity assay kit can be used to quickly assess potential changes in the expression of the gene. The non-radioactive colorimetric
GAPDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble
and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased GAPDH activity.
The kit is stable for at least one year if handled and stored properly.
Kit Components:
GAPDH Assay Solution: 5 ml, store at -20°C or -80°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, Glucose Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay,
G6PD Enzyme Assay
Citation:
Giulivi et al
Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia-susceptible R163C knock-in mice
J Biol Chem, 286:99-113, 2011
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Biomedical Research Service
& Clinical Application
& Clinical Application
Tissue extracts were prepared from
1-month old hamsters, and assayed by
GAPDH Assay Kit. Enzyme activities were
expressed as units per mg proteins using a
purified GAPDH as standard. The study
shows that the liver exhibits a much higher
GAPDH enzyme activity than the heart.
1-month old hamsters, and assayed by
GAPDH Assay Kit. Enzyme activities were
expressed as units per mg proteins using a
purified GAPDH as standard. The study
shows that the liver exhibits a much higher
GAPDH enzyme activity than the heart.
