Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit                                                                                                          Price

Product Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme mediating the conversion of
glyceraldehyde-3-phosphate to 1,3-bisphosphate glycerate with the production of NADH. Although this house-keeping gene is often used as
internal control for RNA and protein analysis, expression of the gene can be altered under certain cell growth and differentiation conditions. In
addition, a switch from aerobic metabolism to anaerobic glycolysis may also be accompanied by a change in GAPDH expression. The GAPDH
enzyme activity assay kit can be used to quickly assess potential changes in the expression of the gene. The non-radioactive colorimetric
GAPDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and
exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased GAPDH activity. The
kit is stable for at least one year if handled and stored properly.

Kit Components:

GAPDH Assay Solution: 5 ml, store at -20°C or -80°C                                 
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: HK Enzyme Assay, Glucose Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay,
G6PD Enzyme Assay

Citation:
Giulivi et al
Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia-susceptible R163C knock-in mice
J Biol Chem, 286:99-113, 2011

Missihoun et al
Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of
delta-sarcoglycan-null cardiomyopathy.
Molecular and Cellular Biochemistry 321:45-52, 2009

Gynecol Oncol, 107:450-457, 2007

J Orthopedic Res, 28:914-920, 2010
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Protein extracts were prepared from 1-month
old hamsters, and assayed by GAPDH
Assay Kit. Enzyme activities were expressed
as units per mg proteins using a purified
GAPDH as standard. The study shows that
the liver exhibits a much higher GAPDH
enzyme activity than the heart.
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