Glutamate Dehydrogenase (GLDH) Assay Kit Price
Product Description: Glutamate dehydrogenase (GLDH), an important mitochondrial matrix enzyme integrating carbon and nitrogen metabolism,
catalyzes the reversible oxidative deamination of L-glutamate into free ammonia and alpha-ketoglutarate in all tissues. Since the forward reaction
generates a TCA cycle intermediate, GLDH is involved in energy homeostasis. In addition, kidney GLDH participates in acid/base balance of blood
by release of ammonia. In clinical diagnostics, increased circulating levels of GLDH signal toxic or hypoxic liver damage. Our non-radioactive
colorimetric GLDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is
water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased
GLDH activity. The assay is simple and non-radioactive. Each kit is sufficient for 200 microassays. The assay solution should be kept in aliquots at
-80ºC, and will remain stable indefinitely at -80ºC.
Kit Components:
GLDH Assay Solution: 10 ml, store at -20ºC or -80ºC (for 200 assays)
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: L-Glutamate Assay
Product Description: Glutamate dehydrogenase (GLDH), an important mitochondrial matrix enzyme integrating carbon and nitrogen metabolism,
catalyzes the reversible oxidative deamination of L-glutamate into free ammonia and alpha-ketoglutarate in all tissues. Since the forward reaction
generates a TCA cycle intermediate, GLDH is involved in energy homeostasis. In addition, kidney GLDH participates in acid/base balance of blood
by release of ammonia. In clinical diagnostics, increased circulating levels of GLDH signal toxic or hypoxic liver damage. Our non-radioactive
colorimetric GLDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is
water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased
GLDH activity. The assay is simple and non-radioactive. Each kit is sufficient for 200 microassays. The assay solution should be kept in aliquots at
-80ºC, and will remain stable indefinitely at -80ºC.
Kit Components:
GLDH Assay Solution: 10 ml, store at -20ºC or -80ºC (for 200 assays)
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: L-Glutamate Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Liver and heart tissue extracts were
assayed by GLDH Assay Kit. Enzyme
activities were expressed as units per mg
standard. The study shows that the liver
exhibits a higher GLDH enzyme activity
than the heart.
assayed by GLDH Assay Kit. Enzyme
activities were expressed as units per mg
standard. The study shows that the liver
exhibits a higher GLDH enzyme activity
than the heart.
