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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Product Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a
glycolytic enzyme mediating the conversion of glyceraldehyde-3-phosphate to
1,3-bisphosphate glycerate with the production of NADH. Although this house-keeping
gene is often used as internal control for RNA and protein analysis, expression of the
gene can be altered under certain cell growth and differentiation conditions. In
addition, a switch from aerobic metabolism to anaerobic glycolysis may also be
accompanied by a change in GAPDH expression. The GAPDH enzyme activity assay kit
can be used to quickly assess potential changes in the expression of the gene. The
non-radioactive colorimetric GAPDH assay is based on the reduction of the tetrazolium
salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and
exhibits an absorption maximum at 492 nm. The intensity of the red color formed is
increased in the presence of increased GAPDH activity. The kit is stable for at least
one year if handled and stored properly. The product is for research use only.
Biomedical Research Service
