GST Antibody Price
BACKGROUND: The immunogen glutathione S-transferase (GST) protein is encoded by the parasitic Schistosoma japonicum GST gene. Many
recombinant proteins are expressed as GST-fusion proteins to facilitate purification or analysis of protein interaction. The parasite GST protein shows
~60% homogy with class Mu isozymes of mammalian GST (EC 2.5.1.18) 2. GST catalyzes the conjugation of reduced glutathione to electrophiles on
many substrates, thus detoxifying endogenous compounds such as lipid peroxides. The polyclonal antibody was produced by immunizing rabbits with
bacterially expressed full-length GST (26 kDa). The product is for research use only.
BACKGROUND: The immunogen glutathione S-transferase (GST) protein is encoded by the parasitic Schistosoma japonicum GST gene. Many
recombinant proteins are expressed as GST-fusion proteins to facilitate purification or analysis of protein interaction. The parasite GST protein shows
~60% homogy with class Mu isozymes of mammalian GST (EC 2.5.1.18) 2. GST catalyzes the conjugation of reduced glutathione to electrophiles on
many substrates, thus detoxifying endogenous compounds such as lipid peroxides. The polyclonal antibody was produced by immunizing rabbits with
bacterially expressed full-length GST (26 kDa). The product is for research use only.
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Biomedical Research Service
& Clinical Application
& Clinical Application
Recombinant GST was
overexpressed in bacteria
(BL21 strain) and purified
by glutathione affinity
chromatography. One ug of
purified GST was analyzed
by SDS-PAGE and Western
blotting using the GST
antibody (1,000 fold
dilution).
overexpressed in bacteria
(BL21 strain) and purified
by glutathione affinity
chromatography. One ug of
purified GST was analyzed
by SDS-PAGE and Western
blotting using the GST
antibody (1,000 fold
dilution).