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Gel Shift Assay Kit

Product Description: Gel shift assay, also known as gel retardation assay or electrophoretic
mobility shift assay (EMSA), is widely used for the detection of DNA/RNA-protein complexes.
Gel shift assays performed with total or nuclear protein extracts require the use of nonspecific
DNA competitors, usually polyanion polymers, to sequester undesired DNA-protein
interaction. The choice of nonspecific competitors however can be tricky since all
DNA-binding proteins exhibit different intrinsic binding specificities and affinities which may be
inadvertently attenuated by the presence of the nonspecific competitors (Nucl. Acids Res.
20:140,1992). In addition, salt concentrations in the binding buffer may differentially affect
DNA-protein and protein-protein interactions (Mol. Cell. Biol. 11:5090,1991). These factors, if
not properly identified and controlled, may lead to irreproducible results. It is thus of benefit to
use a set of dissimilar polyanion competitors and different binding conditions in the screening
of crude protein extracts for novel DNA-binding activities. Our Gel Shift Assay Kit includes a
set of three different polyanion polymers: poly(dI-dC), poly(dG-dC), and sheared salmon
sperm DNA. The binding assay can be performed in two different salt concentrations (10 and
100 mM KCl) to facilitate the detection of different types of higher-order DNA-protein
complexes. Components of the assay kit are stable for at least one year if stored and handled
properly. The product is for research use only.

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