Gel Shift Assay Kit Price
Product Description: Gel shift assay, also known as gel retardation assay or electrophoretic mobility shift assay (EMSA), is widely used for the
detection of DNA/RNA-protein complexes. Gel shift assays performed with total or nuclear protein extracts require the use of nonspecific DNA
competitors, usually polyanion polymers, to sequester undesired DNA-protein interaction. The choice of nonspecific competitors however can be
tricky since all DNA-binding proteins exhibit different intrinsic binding specificities and affinities which may be inadvertently attenuated by the
presence of the nonspecific competitors (Nucl. Acids Res. 20:140,1992). In addition, salt concentrations in the binding buffer may differentially
affect DNA-protein and protein-protein interactions (Mol. Cell. Biol. 11:5090,1991). These factors, if not properly identified and controlled, may lead
to irreproducible results. It is thus of benefit to use a set of dissimilar polyanion competitors and different binding conditions in the screening of
crude protein extracts for novel DNA-binding activities. Our Gel Shift Assay Kit includes a set of three different polyanion polymers: poly(dI-dC),
poly(dG-dC), and sheared salmon sperm DNA. The binding assay can be performed in two different salt concentrations (10 and 100 mM KCl) to
facilitate the detection of different types of higher-order DNA-protein complexes. Components of the assay kit are stable for at least one year if
stored and handled properly. The product is for research use only.
Kit Components:
10x GS Buffer (low): 1 ml, store at 4ºC
10x GS Buffer (high): 1 ml, store at 4ºC
1 mg/ml poly(dI-dC): 0.1 ml, store at 4ºC
1 mg/ml poly(dG-dC): 0.1 ml, store at 4ºC
1 mg/ml Salmon DNA: 0.1 ml, store at 4ºC
GS Loading Solution: 1 ml, store at 4ºC
MSDS: Tris, EDTA, bromophenol blue, DTT
Related kits: Genomic DNA Isolation, Whole Blood DNA Isolation, DNA Transfection, Maxi-Plasmid DNA Purification
Product Description: Gel shift assay, also known as gel retardation assay or electrophoretic mobility shift assay (EMSA), is widely used for the
detection of DNA/RNA-protein complexes. Gel shift assays performed with total or nuclear protein extracts require the use of nonspecific DNA
competitors, usually polyanion polymers, to sequester undesired DNA-protein interaction. The choice of nonspecific competitors however can be
tricky since all DNA-binding proteins exhibit different intrinsic binding specificities and affinities which may be inadvertently attenuated by the
presence of the nonspecific competitors (Nucl. Acids Res. 20:140,1992). In addition, salt concentrations in the binding buffer may differentially
affect DNA-protein and protein-protein interactions (Mol. Cell. Biol. 11:5090,1991). These factors, if not properly identified and controlled, may lead
to irreproducible results. It is thus of benefit to use a set of dissimilar polyanion competitors and different binding conditions in the screening of
crude protein extracts for novel DNA-binding activities. Our Gel Shift Assay Kit includes a set of three different polyanion polymers: poly(dI-dC),
poly(dG-dC), and sheared salmon sperm DNA. The binding assay can be performed in two different salt concentrations (10 and 100 mM KCl) to
facilitate the detection of different types of higher-order DNA-protein complexes. Components of the assay kit are stable for at least one year if
stored and handled properly. The product is for research use only.
Kit Components:
10x GS Buffer (low): 1 ml, store at 4ºC
10x GS Buffer (high): 1 ml, store at 4ºC
1 mg/ml poly(dI-dC): 0.1 ml, store at 4ºC
1 mg/ml poly(dG-dC): 0.1 ml, store at 4ºC
1 mg/ml Salmon DNA: 0.1 ml, store at 4ºC
GS Loading Solution: 1 ml, store at 4ºC
MSDS: Tris, EDTA, bromophenol blue, DTT
Related kits: Genomic DNA Isolation, Whole Blood DNA Isolation, DNA Transfection, Maxi-Plasmid DNA Purification
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Biomedical Research Service
& Clinical Application
& Clinical Application
Myoblasts were transfected with 20 and
500 ng of pMSV-SRF plasmid DNA.
Nuclear extracts were prepared from the
myoblasts and analyzed by the Gel Shift
Assay Kit using a 32P-labeled skeletal
alpha-actin promoter DNA as probe. The
gel shift assay clearly identified the SRF-
and YY1-containing DNA complexes
simultaneously.
500 ng of pMSV-SRF plasmid DNA.
Nuclear extracts were prepared from the
myoblasts and analyzed by the Gel Shift
Assay Kit using a 32P-labeled skeletal
alpha-actin promoter DNA as probe. The
gel shift assay clearly identified the SRF-
and YY1-containing DNA complexes
simultaneously.