Hexokinase Assay Kit Price
Product Description: Hexokinase catalyzes the first step of glycolysis, converting glucose to glucose-6-phosphate. There are four mammalian
hexokinase isoforms (I, II, III, and VI) that differ in tissue distribution and catalytic kinetics. Hexokinase I, II, and III exhibit a low Km for glucose
whereas hexokinase IV (glucokinase) has a Km for glucose 100 times higher than that of other hexokinase. The hexokinase assay solution contains
20 mM glucose, and thus measures the total hexokinase activity of crude cell or tissue extracts. This non-radioactive colorimetric assay is based on
the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption
maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased hexokinase activity. The enzyme assay solution
should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable indefinitely at -80ºC. The product is for research use
only.
Kit Components:
Hexokinase Assay Solution: 10 ml, store at -20ºC or -80ºC
HK Substrate Solution: 0.5 ml, store at 4ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: Glucose Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme
Assay
Product Description: Hexokinase catalyzes the first step of glycolysis, converting glucose to glucose-6-phosphate. There are four mammalian
hexokinase isoforms (I, II, III, and VI) that differ in tissue distribution and catalytic kinetics. Hexokinase I, II, and III exhibit a low Km for glucose
whereas hexokinase IV (glucokinase) has a Km for glucose 100 times higher than that of other hexokinase. The hexokinase assay solution contains
20 mM glucose, and thus measures the total hexokinase activity of crude cell or tissue extracts. This non-radioactive colorimetric assay is based on
the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption
maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased hexokinase activity. The enzyme assay solution
should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable indefinitely at -80ºC. The product is for research use
only.
Kit Components:
Hexokinase Assay Solution: 10 ml, store at -20ºC or -80ºC
HK Substrate Solution: 0.5 ml, store at 4ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: Glucose Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme
Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Protein extracts prepared from human
bone marrow mesenchymal stem cells
(MSC) of early and late passages were
assayed by Hexokinase Assay Kit. Enzyme
activities were expressed as units per mg
proteins using a purified HK as standard.
The study shows that hexokinase activity
is increased upon repeated passages of
MSC in vitro.
bone marrow mesenchymal stem cells
(MSC) of early and late passages were
assayed by Hexokinase Assay Kit. Enzyme
activities were expressed as units per mg
proteins using a purified HK as standard.
The study shows that hexokinase activity
is increased upon repeated passages of
MSC in vitro.