Lactate Dehydrogenase (LDH) Assay Kit                                                                                                                                                       Price

Product Description: Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists
of 4 subunits which may be of 2 different types: M (muscle) and H (heart), also known as A and B respectively. Five different isoenzymes are
therefore possible, depending on the subunit composition (see gel on right). LDH is most often measured to evaluate the presence of tissue or cell
damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to
formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence
of increased LDH activity. The superior sensitivity of the non-radioactive assay kit allows the assay to be completed within 20-30 min with minimal
pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product Lactate Assay Kit. The method is
adaptable to automation.

Kit Components:

LDH Assay Solution: 10 ml, store at -80°C (for 200 assays)
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, Hepes

Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme Assay
Pyruvate Assay, NAD+ assay, L-Lactate Assay

Citation:
Lynch et al
Cell survival under stress is enhanced by a mitochondrial ATP-binding cassette transporter that regulates hemoproteins
Cancer Research 69:5560-5567, 2009


Cancer Res, 66:1684-1693, 2006

J Anim Sci, 87:3124-3133, 2009

Mol Therapy 2:e136,2013
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Mesenchymal stem cells were
treated with 0.1mg/ml AICAR for two
days, following which cell lysates
were prepared for LDH assay. The
study demonstrates that activation of
AMPK results in inhibition of LDH
activity in mesenchymal stem cells.
Mouse heart tissue extracts (10 ug)
were fractionated by a 0.8%
non-denaturing agarose gel,
following which the LDH Assay
Solution was applied to the gel and
incubated at 37C for 1 hour.
Reduction of tetrazolium revealed
the five dark blue LDH bands. Note
that LDH-5 barely migrated through
the well.