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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Lactate Dehydrogenase Assay Kit
Product Description: Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the
interconversion of lactate and pyruvate. It consists of 4 subunits which may be of 2 different
types: M (muscle) and H (heart), formerly known as A and B respectively. Five different
isoenzymes are therefore possible, depending on the subunit composition. LDH is most often
measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric
LDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic
reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm.
The intensity of the red color formed is increased in the presence of increased LDH activity.
The superior sensitivity of the non-radioactive assay kit allows the assay to be completed
within 20-30 min with minimal pipetting steps. Each kit is sufficient for approximately 200
assays using the 96-well format. See related product Lactate Assay Kit. The method is
adaptable to automation.
Biomedical Research Service
