Lactate Dehydrogenase (LDH) Assay Kit Price
Product Description: Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists of 4
subunits which may be of 2 different types: M (muscle) and H (heart), also known as A and B respectively. Five different isoenzymes are therefore
possible, depending on the subunit composition (see gel on right). LDH is most often measured to evaluate the presence of tissue or cell damage. The
non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is
water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased LDH activity.
The superior sensitivity of the non-radioactive assay kit allows the assay to be completed within 20-30 min with minimal pipetting steps. Each kit is
sufficient for approximately 200 assays using the 96-well format. See related product Lactate Assay Kit. The method is adaptable to automation.
Kit Components:
LDH Assay Solution: 10 ml, store at -20°C or -80°C (for 200 assays)
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme Assay
Product Description: Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists of 4
subunits which may be of 2 different types: M (muscle) and H (heart), also known as A and B respectively. Five different isoenzymes are therefore
possible, depending on the subunit composition (see gel on right). LDH is most often measured to evaluate the presence of tissue or cell damage. The
non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is
water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased LDH activity.
The superior sensitivity of the non-radioactive assay kit allows the assay to be completed within 20-30 min with minimal pipetting steps. Each kit is
sufficient for approximately 200 assays using the 96-well format. See related product Lactate Assay Kit. The method is adaptable to automation.
Kit Components:
LDH Assay Solution: 10 ml, store at -20°C or -80°C (for 200 assays)
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
< LDH-5 (M4)
< LDH-4 (H1M3)
< LDH-3 (H2M2)
< LDH-2 (H3M1)
< LDH-1 (H4)
< LDH-4 (H1M3)
< LDH-3 (H2M2)
< LDH-2 (H3M1)
< LDH-1 (H4)
Mouse heart tissue extracts (10 ug
proteins per lane) were fractionated by
0.8% non-denaturing agarose gel, following
which the LDH assay solution was applied
to the gel and incubated at 37C for 1 hour.
Reduction of INT revealed the five bright
red LDH complexes. Note that LDH-5
barely migrated through the well.
proteins per lane) were fractionated by
0.8% non-denaturing agarose gel, following
which the LDH assay solution was applied
to the gel and incubated at 37C for 1 hour.
Reduction of INT revealed the five bright
red LDH complexes. Note that LDH-5
barely migrated through the well.