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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Beta-Galactosidase Assay Kit
Product Description: The bacterial enzyme b-galactosidase (b-Gal) is a widely used reporter
enzyme, the activity of which can be conveniently measured spectrophotometrically (J. Med.
Chem. 7:574,1964). The b-Gal assay kit utilizes the spectroscopically active compound
o-nitrophenyl-b-D-galactopyranoside (ONPG) for quantitative measurements of b-Gal activity
in cells (higher eukaryotes, yeast, or bacteria) transfected or transformed with b-Gal reporter
DNA (see b-Galactosidase Staining Kit). The enzyme converts ONPG to o-nitrophenol, which
exhibits an extinction coefficient of 4,500 M-1cm-1 at 420 nm. Each assay kit includes 25 ml
of optimized ONPG Substrate Solution sufficient for 250 assays in microplate formats. Cell
Lysis Solution is compatible with luciferase (see Luciferase Assay Kit) and CAT enzyme
assays permitting flexibility and maximum sensitivity. Substrate is stable for at least one year
if handled and stored properly. The method is adaptable to automation.
Biomedical Research Service
