LacZ Staining Kit Price
Product Description: The bacterial enzyme beta-galactosidase is a widely used reporter enzyme, the activity of which can be conveniently
measured spectrophotometrically (J. Med. Chem. 7:574,1964). The beta-Galactosidase Staining Kit is based on the histochemical substrate 5-
bromo-4-chloro-3-indolyl-b-D-galactopyranoside (Xgal) for in situ staining of transgenic tissues or monolayer cells transiently or stably expressing
E. coli beta-galactosidase (see LacZ Assay Kit). The cells expressing b-galactosidase stain blue after mild fixation and substrate exposure. The
staining kit requires minimal steps for staining the enzyme. This method provides rapid information regarding exogenous expression of beta-
galactosidase in animal tissue and transfection efficiency in cell culture. Note that a 4% paraformaldehyde solution is required for cell fixation, but
is not included in the kit.
10x LacZ Staining Buffer: 15 ml, store at 4ºC
Ferricyanide: 10 ml, store at 4ºC
Ferrocyanide: 10 ml, store at 4ºC
Xgal: 4 x 1 ml, store at -20ºC
MSDS: potasium ferricyanide, potasium ferrocyanide, Xgal, formamide, paraformaldehyde
Related kits: LacZ Assay, ALP Assay, Luciferase Assay, Osteogenesis Assay, Chondrogenesis Assay
Lin et al
Cellular toxicity induced by SRF-mediated transcriptional squelching.
Toxicol Sci. 96:83-91, 2007
Biomedical Research Service
& Clinical Application
The stained cells exhibit dark-blue nuclei
with a light-blue cytoplasm.