Beta-Galactosidase (GAL) Staining Kit Price
Product Description: The bacterial enzyme b-galactosidase (b-Gal) is a widely used reporter enzyme, the activity of which can be conveniently
measured spectrophotometrically (J. Med. Chem. 7:574,1964). The b-Gal Staining Kit is based on the histochemical substrate
5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (Xgal) for in situ staining of transgenic tissues or monolayer cells transiently or stably
expressing b-Gal (see b-Galactosidase Assay Kit). The cells expressing b-Gal stain blue after mild fixation and substrate exposure. The staining
kit requires minimal steps for staining b-Gal. This method provides rapid information regarding exogenous expression of b-Gal in animal tissue and
transfection efficiency in cell culture. Note that a 4% paraformaldehyde solution is required for cell fixation, but is not included in the kit.
X-gal Buffer: 150 ml, store at 4ºC
Ferricyanide: 10 ml, store at 4ºC
Ferrocyanide: 10 ml, store at 4ºC
X-gal: 4 ml, store at -20ºC
MSDS: potasium ferricyanide, potasium ferrocyanide, Xgal, formamide, paraformaldehyde
Related kits: beta-Galactosidase (LacZ) Assay, ALP Assay, Luciferase Assay, Osteogenesis Assay, Chondrogenesis Assay
Lin et al
Cellular toxicity induced by SRF-mediated transcriptional squelching.
Toxicol Sci. 96:83-91, 2007
Biomedical Research Service
& Clinical Application
The stained cells exhibit dark-blue nuclei
with a light-blue cytoplasm.