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Beta-Galactosidase Staining Kit

Product Description: The bacterial enzyme b-galactosidase (b-Gal) is a widely used reporter
enzyme, the activity of which can be conveniently measured spectrophotometrically (J. Med.
Chem. 7:574,1964). The b-Gal Staining Kit is based on the histochemical substrate
5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (Xgal) for in situ staining of transgenic
tissues or monolayer cells transiently or stably expressing b-Gal (see b-Galactosidase Assay
Kit). The cells expressing b-Gal stain blue after mild fixation and substrate exposure. The
staining kit requires minimal steps for staining b-Gal. This method provides rapid information
regarding exogenous expression of b-Gal in animal tissue and transfection efficiency in cell
culture. Note that a 4% paraformaldehyde solution is required for cell fixation, but is not
included in the kit.

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