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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Lactate Assay Kit
Product Description: Production of lactate mediated by the enzyme lactate dehydrogenase
(LDH) is increased under hypoxic or anaerobic conditions. Measurement of cellular lactate
concentration is thus of interest in the study of cellular as well as animal physiology,
providing an important index of the balance between tissue oxygen demand and utilization.
Our Lactate Assay Kit is based on the reduction of the tetrazolium salt INT in a
NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an
absorption maximum at 492 nm. Since the intensity of the red color formed is proportional to
the lactate concentration, the assay, using a set of lactate standards, can measure the
concentration of lactate released to the culture medium, circulation, or urine in a quantitative
manner. The non-radioactive assay kit allows the assay to be completed in one step within
30-60 min. The kit can be used for clinical samples such as serum, plasma, and urine (no
TCA treatment is required). Each kit is sufficient for 200 (A-108S) or 400 (A-108L) assays
using the 96-well format. The assay can be scaled up for spectrophotometric measurements.
The only equipment required is a spectrophotometer or a microplate reader. See LDH Assay
Kit. The method is adaptable to automation.
Biomedical Research Service
