L-Lactate Assay Kit                                                                                                                                                                                         Price

Product Description: Production of L-lactate mediated by the enzyme lactate dehydrogenase (LDH) is increased under hypoxic or anaerobic
conditions. Measurement of cellular lactate concentration is thus of interest in the study of cellular as well as animal physiology, providing an
important index of the balance between tissue oxygen demand and utilization (see assay on right). Our Lactate Assay Kit is based on the
reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption
maximum at 492 nm. Since the intensity of the red color formed is proportional to the lactate concentration, the assay, using a set of lactate
standards, can measure the concentration of lactate in the cell and released to the culture medium, circulation, or urine in a quantitative
manner. The non-radioactive assay kit allows the assay to be completed in one step within 30-60 min. The kit can be used for clinical samples
such as serum, plasma, and urine. Intracellular lactate can be extracted by a simple boiling method and measured by the assay kit. Each kit is
sufficient for 200 (A-108S) or 400 (A-108L) assays using the 96-well format. The assay can be scaled up for spectrophotometric measurements.
The only equipment required is a spectrophotometer or a microplate reader. The method is adaptable to automation.

Kit Components:

L-Lactate Assay Solution: 10 ml for A-108S or 20 ml for A-108L; store at -80°C
1 mM L-Lactate Standard: 0.5 ml; store at -20°C
50% PEG-8000 Solution: 15 ml; store at 4°C

MSDS: DMSO, INT, acetic acid, Hepes

Related kits: D-Lactate Assay, LDH Assay, PDH Assay, ATP Assay, Pyruvate Assay, NAD+ Assay

Citation:
Gordon et al
Brain metabolism dictates the polarity of astrocyte control over arterioles
Nature, 456, 745-749, 2008
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Biomedical Research Service
& Clinical Application
HEK293 cells constitutively expressing bcl-2
show altered glucose metabolism. Culture
media were diluted 10 fold and mixed with
the L-Lactate Assay Solution. L-lactate
concentrations were deduced from a lactate
standard plot. The assay reveals that lactate
production by the Bcl-2 expressor is
reduced compared to the parental HEK293
cells.
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