Lipid Peroxidation Assay Kit                                                                                                                                                                            Price

Product Description: Cells exposed to oxidative stress accumulate a complex profile of aldehydes which are derived from the decomposition of
lipid hydroperoxides (Free Radical Biology & Medicine 7:197,1989). In contrast to free radicals, also caused by oxidative stress, the aldehydes
are more stable and tend to diffuse over large distances both intracellularly and extracellularly. Thus, profound cytotoxic and genotoxic effects
are associated with these aldehyde products, and the determination of aldehydes is of wide interests. Malondialdehyde (MDA) and MDA-like
substances are by far the most abundant lipid peroxidation products. High levels of MDA and MDA-like substances are produced during the
oxidation of low density lipoprotein. Although HPLC can be used to measure the production of these lipid peroxidation products, the analysis of
MDA by the classical TBA (2-thiobarbituric acid) test remains a simple, reliable, and useful method applicable in most if not every laboratory (J.
Lipid Res. 28:495,1987). Our Lipid Peroxidation Assay Kit is formulated based on the TBA method. The kit is stable for one year if stored and
handled properly. The product is for research use only.

Kit Components:

TBA: 0.75 g, store at room temperature
MDA Assay Solution: 100 ml, store at room temperature

MSDS: NaOH, 2-thiobarbituric acid

Related kits: Cell Injury Assay, Peroxide Assay, Nitric Oxide Assay, Cell Viability Assay
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Biomedical Research Service
                   & Clinical Application
Normal hamster (F1B strain) and
dystrophic hamster (TO2 strain) were
used in the study. TO2 hamster received
intramuscular injection of mesenchymal
stem cells, and the group is indicated as
TO2/MSC. Hamstring muscles from
normal F1B, saline- and MSC-injected TO2
hamsters were harvested after one month
for MDA analysis. The study shows the
dystrophic muscle contains elevated
levels of MDA, which could be reduced to
normal levels by the MSC therapy.