Luciferase Assay Kit Price
Product Description: Luciferase encoded by the firefly luciferase gene is widely used as a sensitive reporter enzyme for the study of
transcriptional regulation. The enzyme catalyzes, in the presence of ATP (see ATP Assay Kit), the oxidation of luciferin with concomitant emission
of yellow-green light, which can be conveniently measured by scintillation counters or luminometers. Light emission peaks in several seconds at
560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance and decay of the light flash require
consistent timing of the light measurement to obtain reliable data. This provides the basis for an assay system many times more sensitive than the
b-galactosidase (GAL) or other reporter gene systems (PNAS USA 82:7870,1984 & Mol. Cell. Biol. 7:725,1987). The luciferase-based reporter
assay is thus well suited for those cell systems exhibiting low transfection efficiency. The kit is sufficient for 200 assays using 0.1 ml of Luciferase
Substrate per assay. Reconstituted Luciferase Substrate solution if stored in aliquots at -75°C is stable for at least one year. Repeated
freeze-thaw cycles should be avoided. The product is for research use only.
Kit Components:
Luciferase Assay Solution: 20 ml, store at 4°C
Luciferase Assay Substrate: 1 vial; store IN ALIQUOTS at -80°C after reconstitution
10x Lysis Solution: 25 ml, store at 4°C
MSDS: TX-100, Tris, luciferin, EDTA, DTT
Related kits: beta-Galactosidase (LacZ) Assay, beta-Galactosidase (LacZ) Staining, ALP Assay
Product Description: Luciferase encoded by the firefly luciferase gene is widely used as a sensitive reporter enzyme for the study of
transcriptional regulation. The enzyme catalyzes, in the presence of ATP (see ATP Assay Kit), the oxidation of luciferin with concomitant emission
of yellow-green light, which can be conveniently measured by scintillation counters or luminometers. Light emission peaks in several seconds at
560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance and decay of the light flash require
consistent timing of the light measurement to obtain reliable data. This provides the basis for an assay system many times more sensitive than the
b-galactosidase (GAL) or other reporter gene systems (PNAS USA 82:7870,1984 & Mol. Cell. Biol. 7:725,1987). The luciferase-based reporter
assay is thus well suited for those cell systems exhibiting low transfection efficiency. The kit is sufficient for 200 assays using 0.1 ml of Luciferase
Substrate per assay. Reconstituted Luciferase Substrate solution if stored in aliquots at -75°C is stable for at least one year. Repeated
freeze-thaw cycles should be avoided. The product is for research use only.
Kit Components:
Luciferase Assay Solution: 20 ml, store at 4°C
Luciferase Assay Substrate: 1 vial; store IN ALIQUOTS at -80°C after reconstitution
10x Lysis Solution: 25 ml, store at 4°C
MSDS: TX-100, Tris, luciferin, EDTA, DTT
Related kits: beta-Galactosidase (LacZ) Assay, beta-Galactosidase (LacZ) Staining, ALP Assay
 | |  | |  | |  | |  | |  | |  | |  | |  | |  | |  |
Biomedical Research Service
& Clinical Application
& Clinical Application
N-terminal 90 and 138 amino acids of the
transcription factor YY1 are fused in-frame
with the DNA-binding domain of GAL4. The
trans-activation activity of the GAL4 fusion
protein was tested using a luciferase
reporter construct regulated by a promoter
element driven by multimerized GAL4 binding
sites. The cotransfection study reveals the
transcriptional activation domain of YY1.
transcription factor YY1 are fused in-frame
with the DNA-binding domain of GAL4. The
trans-activation activity of the GAL4 fusion
protein was tested using a luciferase
reporter construct regulated by a promoter
element driven by multimerized GAL4 binding
sites. The cotransfection study reveals the
transcriptional activation domain of YY1.
