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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Luciferase Assay Kit
Product Description: Luciferase encoded by the firefly luciferase gene is widely used as a
sensitive reporter enzyme for the study of transcriptional regulation. The enzyme catalyzes, in
the presence of ATP (see ATP Assay Kit), the oxidation of luciferin with concomitant emission
of yellow-green light, which can be conveniently measured by scintillation counters or
luminometers. Light emission peaks in several seconds at 560 nm when the reaction is
conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance and decay of the
light flash require consistent timing of the light measurement to obtain reliable data. This
provides the basis for an assay system many times more sensitive than the b-galactosidase
(GAL) or other reporter gene systems (PNAS USA 82:7870,1984 & Mol. Cell. Biol.
7:725,1987). The luciferase-based reporter assay is thus well suited for those cell systems
exhibiting low transfection efficiency. The kit is sufficient for 200 assays using 0.1 ml of
Luciferase Substrate per assay. Reconstituted Luciferase Substrate solution if stored in
aliquots at -75°C is stable for at least one year. Repeated freeze-thaw cycles should be
avoided. The product is for research use only.
Biomedical Research Service
