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Maxi-Plasmid DNA Purification Kit

Product Description: Isolation of high-quality plasmid DNA from the gemisch of the E. coli cell
lysate paves the way for many laboratory as well as clinical genetic applications, among which
are clone screening, DNA sequencing, DNA transfection, probe preparation, PCR analysis,
and gene therapy, to name but a few. Although small-scale plasmid purification (mini-prep) is
often sufficient for DNA cloning and sequencing, large-scale (maxi-prep) plasmid purification is
required for gene transfer and gene therapy studies. Our Maxi-Plasmid Isolation Kit is similar
to the Mini-Plasmid Isolation Kit with additional filtration and precipitation steps. Plasmid DNA
can be easily and rapidly purified without the use of phenol. Each Maxi-prep DNA is purified
from 100 - 200 ml of overnight E. coli culture in 90 min with a typical yield of 0.5 – 3 ug DNA
(as determined by plasmid replication origin) per ml of culture. Purified plasmid DNA, typically
with an A260/A280 ratio of ~2, is suitable for restriction digestion, probe preparation, DNA
sequencing, PCR analysis, and transfection. The kit (sufficient for 10 maxi-preps) is stable for
at least one year if handled and stored properly. The product is for research use only.

Biomedical Research Service