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Mini-Plasmid DNA Purification Kit

Product Description: Isolation of high-quality plasmid DNA from the gemisch of the E. coli cell
lysate paves the way for many laboratory as well as clinical genetic applications, among which
are clone screening, DNA sequencing, DNA transfection, probe preparation, PCR analysis,
and gene therapy, to name but a few. Small-scale plasmid purification (mini-prep) is often
used to facilitate clone screening and DNA sequencing. Although many commercial mini-prep
kits are offered, few combines the following three features: simplicity, low cost, and high
quality. We have modified and streamlined the alkaline lysis mini-prep method of Sambrook et
al. (Molecular Cloning, CSH, NY) eliminating the needs for additional RNase treatment and
phenol-chloroform extraction. Plasmid DNA can be easily and rapidly purified from an
overnight E. coli culture in 15 min with a typical yield of 1 – 5 ug DNA per ml of culture. The
DNA is suitable for restriction digestion, probe preparation, and PCR analysis. DNA to be used
for sequencing analysis is subjected to an additional 10-min step of PEG precipitation (PS-4)
to remove any trace amount of RNA. High-quality DNA sequencing ladders can be easily
obtained (almost 1,000 bases resolved per sequencing run) if the plasmid DNA is isolated
from a suitable E. coli host strain. The kit (sufficient for 300 mini-preps) is stable for at least
one year if handled and stored properly. The product is for research use only.

Biomedical Research Service