Nitric Oxide/Nitrite Assay Kit Price
Product Description: Nitric oxide (NO) is a potent endogenous vasodilator, and has a role in neurotransmission, inflammation, thrombosis,
immunity, and control of cell growth and cell death. Interest in measurement of NO increased exponentially with the discovery of these versatile
roles of NO in physiology as well as pathophysiology. Although NO can be measured by diazotization of sulfanilic acid, electron paramagnetic
resonance, met-hemoglobin conversion, and chemiluminescence reactions (FASEB J. &:349,1993), measurement of NO in biological samples is
difficult due to its low level and lability in the presence of oxygen. Nitrite is the major NO byproduct upon reaction with oxygen. Thus, the easiest
method for NO measurement is the standard technique for measuring inorganic nitrite. This assay is based on the observation that the adduct of
nitroxides and sulfanilic acid interacts with N-(1-naphthyl)ethylenediamine, generating a product that is readily monitored by spectrophotometry
(Anal. Biochem. 126:131,1982). Nitrite concentrations can be obtained by comparison to a standard curve performed at the same time. The
assay kit (sufficient for 500 microplate assays) is stable for one year if stored refrigerated and shielded from light. The product is for research
use only.
Kit Components:
NO Assay Solution: 50 ml, store at 4ºC
Sodium Nitrite: 1 g, store at room temperature
MSDS: phosphoric acid, N-(1-naphthyl)ethylenediamine, nitrite, sulfanilic acid
Related kits: Lipid Peroxidation Assay, Peroxide Assay, Cell Injury Assay, Cell Viability Assay
Product Description: Nitric oxide (NO) is a potent endogenous vasodilator, and has a role in neurotransmission, inflammation, thrombosis,
immunity, and control of cell growth and cell death. Interest in measurement of NO increased exponentially with the discovery of these versatile
roles of NO in physiology as well as pathophysiology. Although NO can be measured by diazotization of sulfanilic acid, electron paramagnetic
resonance, met-hemoglobin conversion, and chemiluminescence reactions (FASEB J. &:349,1993), measurement of NO in biological samples is
difficult due to its low level and lability in the presence of oxygen. Nitrite is the major NO byproduct upon reaction with oxygen. Thus, the easiest
method for NO measurement is the standard technique for measuring inorganic nitrite. This assay is based on the observation that the adduct of
nitroxides and sulfanilic acid interacts with N-(1-naphthyl)ethylenediamine, generating a product that is readily monitored by spectrophotometry
(Anal. Biochem. 126:131,1982). Nitrite concentrations can be obtained by comparison to a standard curve performed at the same time. The
assay kit (sufficient for 500 microplate assays) is stable for one year if stored refrigerated and shielded from light. The product is for research
use only.
Kit Components:
NO Assay Solution: 50 ml, store at 4ºC
Sodium Nitrite: 1 g, store at room temperature
MSDS: phosphoric acid, N-(1-naphthyl)ethylenediamine, nitrite, sulfanilic acid
Related kits: Lipid Peroxidation Assay, Peroxide Assay, Cell Injury Assay, Cell Viability Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Human bone marrow mesenchymal stem
cells were infected with adenovirus
expressing LacZ, VEGF164, and VEGF188.
Culture media were collected three days
later, and assayed for nitrite concentrations.
The study shows the longer VEGF isoform
likely causes NOS activation and nitric oxide
release from MSC.
cells were infected with adenovirus
expressing LacZ, VEGF164, and VEGF188.
Culture media were collected three days
later, and assayed for nitrite concentrations.
The study shows the longer VEGF isoform
likely causes NOS activation and nitric oxide
release from MSC.