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Nitric Oxide/Nitrite Assay Kit

Product Description: Nitric oxide (NO) is a potent endogenous vasodilator, and has a role in
neurotransmission, inflammation, thrombosis, immunity, and control of cell growth and cell
death. Interest in measurement of NO increased exponentially with the discovery of these
versatile roles of NO in physiology as well as pathophysiology. Although NO can be measured
by diazotization of sulfanilic acid, electron paramagnetic resonance, met-hemoglobin
conversion, and chemiluminescence reactions (FASEB J. &:349,1993), measurement of NO in
biological samples is difficult due to its low level and lability in the presence of oxygen. Nitrite
is the major NO byproduct upon reaction with oxygen. Thus, the easiest method for NO
measurement is the standard technique for measuring inorganic nitrite. This assay is based
on the observation that the adduct of nitroxides and sulfanilic acid interacts with
N-(1-naphthyl)ethylenediamine, generating a product that is readily monitored by
spectrophotometry (Anal. Biochem. 126:131,1982). Nitrite concentrations can be obtained by
comparison to a standard curve performed at the same time. The assay kit (sufficient for 500
microplate assays) is stable for one year if stored refrigerated and shielded from light. The
product is for research use only.

Biomedical Research Service