Nitric Oxide/Nitrite Assay Kit                                                                                                                                                                        Price

Product Description: Nitric oxide (NO) is a potent endogenous vasodilator, and has a role in neurotransmission, inflammation, thrombosis,
immunity, and control of cell growth and cell death. Interest in measurement of NO increased exponentially with the discovery of these versatile
roles of NO in physiology as well as pathophysiology. Although NO can be measured by diazotization of sulfanilic acid, electron paramagnetic
resonance, met-hemoglobin conversion, and chemiluminescence reactions (FASEB J. &:349,1993), measurement of NO in biological samples is
difficult due to its low level and lability in the presence of oxygen. Nitrite is the major NO byproduct upon reaction with oxygen. Thus, the easiest
method for NO measurement is the standard technique for measuring inorganic nitrite. This assay is based on the observation that the adduct of
nitroxides and sulfanilic acid interacts with N-(1-naphthyl)ethylenediamine, generating a product that is readily monitored by spectrophotometry
(Anal. Biochem. 126:131,1982). Nitrite concentrations can be obtained by comparison to a standard curve performed at the same time. The
assay kit (sufficient for 500 microplate assays) is stable for one year if stored refrigerated and shielded from light.

Kit Components:

NO Assay Solution:         50 ml, store at 4ºC
Sodium Nitrite:                 1 g, store at room temperature

MSDS: phosphoric acid, N-(1-naphthyl)ethylenediamine, nitrite, sulfanilic acid

Related kits: Lipid Peroxidation Assay, Peroxide Assay, Cell Injury Assay, Cell Viability Assay, Free Thiol Assay

Citation:
Kiang et al
17-DMAG diminishes hemorrhage-induced small intestine injury by elevating Bcl-2 protein and inhibiting iNOS pathway, TNF-α increase, and
caspase-3 activation.
Cell Bioscience 1:21, 2011
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& Clinical Application
Mesenchymal stem cells were infected with
the indicated adenoviruses. Culture media
were collected three days later, and assayed
for nitrite concentrations. The study shows the
longer VEGF isoform caused increased nitric
oxide release from MSC.
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