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Osteogenesis Assay Kit

Product Description: The osteogenesis assay kit is based on the alkaline phosphatase
(ALP)-mediated conversion of p-nitrophenol phosphate to nitrophenol in an alkaline buffer.
Nitrophenol exhibits an absorption maximum at 405-415 nm. The assay can also be used to
examine cell differentiation such as osteogenesis, which is associated with increased
expression of ALP. Repeated freeze-thaw cycles of the assay solution should be avoided. The
enzyme assay system can be completed in as short as 10 minutes. The method is adaptable
to automation.

Biomedical Research Service