Osteogenesis Assay Kit                                                                                                                                                                                Price

Product Description: The osteogenesis assay kit is based on the alkaline phosphatase (ALP)-mediated conversion of p-nitrophenol phosphate
to nitrophenol in an alkaline buffer. Nitrophenol exhibits an absorption maximum at 405-415 nm. The assay can also be used to examine cell
differentiation such as osteogenesis, which is associated with increased expression of ALP. Repeated freeze-thaw cycles of the assay solution
should be avoided. The enzyme assay system can be completed in as short as 10 minutes. The method is adaptable to automation.

Kit Components:

10x Cell Lysis Solution: 25 ml, store at RT
ALP Assay Solution: 50 ml, store in aliquots at -20ºC

MSDS: p-nitrophenol phosphate, Tris

Related kits: Chondrogenesis Assay, Adipogenesis Assay, Cell Proliferation Assay

Citation:
Leiker et al
Assessment of a nuclear affinity labeling method for tracking implanted mesenchymal stem cells
Cell Transplantation 17:911-922, 2008
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Biomedical Research Service
& Clinical Application
Measuring the activities of alkaline
phosphatase (Alk) and beta-galactosidase
(Gal) in crude cell extracts. Early and late
passage bone marrow mesenchymal stem
cells were solubilized in a lysis Alk and Gal
assay solutions. The assays demonstrate
differential changes in enzyme activities
associated with adult stem cell senescence
in culture.
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