Osteogenesis Assay Kit Price
Product Description: The osteogenesis assay kit is based on the alkaline phosphatase (ALP)-mediated conversion of p-nitrophenol phosphate
to nitrophenol in an alkaline buffer. Nitrophenol exhibits an absorption maximum at 405-415 nm. The assay can also be used to examine cell
differentiation such as osteogenesis, which is associated with increased expression of ALP. Repeated freeze-thaw cycles of the assay solution
should be avoided. The enzyme assay system can be completed in as short as 10 minutes. The method is adaptable to automation.
Kit Components:
10x Cell Lysis Solution: 25 ml, store at RT
ALP Assay Solution: 50 ml, store in aliquots at -20ºC
MSDS: p-nitrophenol phosphate, Tris
Related kits: Chondrogenesis Assay, Adipogenesis Assay, Cell Proliferation Assay
Product Description: The osteogenesis assay kit is based on the alkaline phosphatase (ALP)-mediated conversion of p-nitrophenol phosphate
to nitrophenol in an alkaline buffer. Nitrophenol exhibits an absorption maximum at 405-415 nm. The assay can also be used to examine cell
differentiation such as osteogenesis, which is associated with increased expression of ALP. Repeated freeze-thaw cycles of the assay solution
should be avoided. The enzyme assay system can be completed in as short as 10 minutes. The method is adaptable to automation.
Kit Components:
10x Cell Lysis Solution: 25 ml, store at RT
ALP Assay Solution: 50 ml, store in aliquots at -20ºC
MSDS: p-nitrophenol phosphate, Tris
Related kits: Chondrogenesis Assay, Adipogenesis Assay, Cell Proliferation Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Measuring the activities of alkaline
phosphatase (Alk) and beta-galactosidase
(Gal) in crude cell extracts. Early and late
passage bone marrow mesenchymal
stem cells were solubilized in a lysis
buffer, and cell lysates were mixed with
Alk and Gal assay solutions. The assays
demonstrate differential changes in
enzyme activities associated with adult
stem cell senescence in culture.
phosphatase (Alk) and beta-galactosidase
(Gal) in crude cell extracts. Early and late
passage bone marrow mesenchymal
stem cells were solubilized in a lysis
buffer, and cell lysates were mixed with
Alk and Gal assay solutions. The assays
demonstrate differential changes in
enzyme activities associated with adult
stem cell senescence in culture.