Pyruvate Dehydrogenase Assay Kit Price
Product Description: Pyruvate is the end product of aerobic glycolysis and is at an important metabolic crossroad. The pyruvate dehydrogenase
multi-enzyme complex (PDH) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA in a series of reactions mediated by the E1 subunit
(pyruvate dehydrogenase), E2 subunit (dihydrolipoyl transacetylase), and E3 subunit (dihydrolipoyl dehydrogenase). The reactions of the PDH
complex serve to interconnect the metabolic pathways of glycolysis, gluconeogenesis and fatty acid synthesis to the Krebs (TCA) cycle. As a
consequence, the PDH complex is highly regulated by a variety of allosteric effectors and by covalent modification. The importance of the PDH
complex in metabolic regulation is evident from the fact that although diseases associated with deficiencies of the PDH complex have been observed,
affected individuals often do not survive to maturity. This non-radioactive colorimetric PDH assay is based on the reduction of the tetrazolium salt INT
in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red
color formed is increased in the presence of increased PDH activity. The kit is stable for at least one year under proper storage and handling
conditions. The product is for research use only.
Kit Components:
PDH Assay Solution: 5 ml, store at -20ºC or -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, Glucose Assay, G6PD Enzyme Assay
Product Description: Pyruvate is the end product of aerobic glycolysis and is at an important metabolic crossroad. The pyruvate dehydrogenase
multi-enzyme complex (PDH) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA in a series of reactions mediated by the E1 subunit
(pyruvate dehydrogenase), E2 subunit (dihydrolipoyl transacetylase), and E3 subunit (dihydrolipoyl dehydrogenase). The reactions of the PDH
complex serve to interconnect the metabolic pathways of glycolysis, gluconeogenesis and fatty acid synthesis to the Krebs (TCA) cycle. As a
consequence, the PDH complex is highly regulated by a variety of allosteric effectors and by covalent modification. The importance of the PDH
complex in metabolic regulation is evident from the fact that although diseases associated with deficiencies of the PDH complex have been observed,
affected individuals often do not survive to maturity. This non-radioactive colorimetric PDH assay is based on the reduction of the tetrazolium salt INT
in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red
color formed is increased in the presence of increased PDH activity. The kit is stable for at least one year under proper storage and handling
conditions. The product is for research use only.
Kit Components:
PDH Assay Solution: 5 ml, store at -20ºC or -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, Glucose Assay, G6PD Enzyme Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
Heart tissue homogenates were prepared
from normal (F1B strain) and
cardiomyopathic (TO2 strain) hamsters at
1 and 4 months. Pelleted membrane
fractions were used for PDH activity
assay. The study shows that the
cardiomyopathic hamster heart fails to
exhibit an age-dependent increase in PDH
activity.
from normal (F1B strain) and
cardiomyopathic (TO2 strain) hamsters at
1 and 4 months. Pelleted membrane
fractions were used for PDH activity
assay. The study shows that the
cardiomyopathic hamster heart fails to
exhibit an age-dependent increase in PDH
activity.