Pyruvate Dehydrogenase (PDH) Assay Kit                                                                                                                                                    Price

Product Description: Pyruvate is the end product of aerobic glycolysis and is at an important metabolic crossroad. The pyruvate dehydrogenase
multi-enzyme complex (PDH) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA in a series of reactions mediated by the E1 subunit
(pyruvate dehydrogenase), E2 subunit (dihydrolipoyl transacetylase), and E3 subunit (dihydrolipoyl dehydrogenase). The reactions of the PDH
complex serve to interconnect the metabolic pathways of glycolysis, gluconeogenesis and fatty acid synthesis to the Krebs (TCA) cycle. As a
consequence, the PDH complex is highly regulated by a variety of allosteric effectors and by covalent modification. The importance of the PDH
complex in metabolic regulation is evident from the fact that although diseases associated with deficiencies of the PDH complex have been
observed, affected individuals often do not survive to maturity. This non-radioactive colorimetric PDH assay is based on the reduction of the
tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The
intensity of the red color formed is increased in the presence of increased PDH activity. The kit is stable for at least one year under proper storage
and handling conditions.

Kit Components:

PDH Assay Solution: 5 ml, store at -20ºC or -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, Glucose Assay, G6PD Enzyme
Assay
Pyruvate Assay, NAD+ Assay

Citation:
Missihoun et al
Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of
delta-sarcoglycan-null cardiomyopathy.
Molecular and Cellular Biochemistry 321:45-52, 2009


Deng et al
Protein Kinase B (PKB/AKT1) Formed Signaling Complexes with Mitochondrial Proteins and Prevented Glycolytic Energy Dysfunction in Cultured
Cardiomyocytes During Ischemia-Reperfusion Injury
Endocrinology 155:1618-1628, 2014


Martin et al
Androgen deprivation leads to increased carbohydrate metabolism and hexokinase 2-mediated survival in Pten/Tp53-deficient prostate cancer
Oncogene
36:525-533, 2017
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Biomedical Research Service
& Clinical Application
PDH assays were performed using heart
extracts prepared from normal (F1B strain)
and cardiomyopathic (TO2 strain) hamsters
at 1 and 4 months.  Pelleted membrane
fractions were used for PDH activity assay.
The study shows that the cardiomyopathic
hamster heart fails to exhibit an
age-dependent increase in PDH activity.
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