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Pyruvate Dehydrogenase Assay Kit

Product Description: Pyruvate is the end product of aerobic glycolysis and is at an important
metabolic crossroad. The pyruvate dehydrogenase multi-enzyme complex (PDH) catalyzes the
oxidative decarboxylation of pyruvate to acetyl-CoA in a series of reactions mediated by the E1
subunit (pyruvate dehydrogenase), E2 subunit (dihydrolipoyl transacetylase), and E3 subunit
(dihydrolipoyl dehydrogenase). The reactions of the PDH complex serve to interconnect the
metabolic pathways of glycolysis, gluconeogenesis and fatty acid synthesis to the Krebs (TCA)
cycle. As a consequence, the PDH complex is highly regulated by a variety of allosteric
effectors and by covalent modification. The importance of the PDH complex in metabolic
regulation is evident from the fact that although diseases associated with deficiencies of the
PDH complex have been observed, affected individuals often do not survive to maturity. This
non-radioactive colorimetric PDH assay is based on the reduction of the tetrazolium salt INT in
a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an
absorption maximum at 492 nm. The intensity of the red color formed is increased in the
presence of increased PDH activity. The kit is stable for at least one year under proper
storage and handling conditions. The product is for research use only.

Biomedical Research Service