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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Phosphate Assay Kit
Product Description: Quantification of inorganic as well as organic phosphates (such as
phospholipids, phosphoproteins, and nucleid acids) is of interest in many biological studies.
Phosphatase activity, for instance, can be derived from such an analysis after co-incubation
of a phosphatase and a specific phosphopeptide substrate (J. Cell. Biochem. 77:445,2000).
Similarly, an assay for phospholipase activity can be performed after hydrolysis of
phospholipids by phospholipases. Although various methods can be employed to measure
phosphates, non-radioactive colorimetric protocols suitable for high-throughput analysis are
the methods of choice in most laboratories. Perhaps the most sensitive colorimetric method
for phosphate assay is based on complex formation of phosphomolybdate with the basic dye
Malachite Green (Anal. Biochem. 161:45,1987 & 271:29,1999). Our Phosphate Assay Kit
(sufficient for 400 assays) is formulated to maximize the sensitivity and stability of the
dye-phosphomolybdate reaction. The kit is stable for at least one year if handled and stored
properly. The assay, compatible with microplate formats, is simple and can be finished in 15 -
20 min. Reaction product is quantified by absorbance at 600-660 nm.
Biomedical Research Service
