Phosphate Assay Kit Price
Product Description: Quantification of inorganic as well as organic phosphates (such as phospholipids, phosphoproteins, and nucleid acids) is of
interest in many biological studies. Phosphatase activity, for instance, can be derived from such an analysis after co-incubation of a phosphatase
and a specific phosphopeptide substrate (J. Cell. Biochem. 77:445,2000). Similarly, an assay for phospholipase activity can be performed after
hydrolysis of phospholipids by phospholipases. Although various methods can be employed to measure phosphates, non-radioactive colorimetric
protocols suitable for high-throughput analysis are the methods of choice in most laboratories. Perhaps the most sensitive colorimetric method for
phosphate assay is based on complex formation of phosphomolybdate with the basic dye Malachite Green (Anal. Biochem. 161:45,1987 &
271:29,1999). Our Phosphate Assay Kit (sufficient for 400 assays) is formulated to maximize the sensitivity and stability of the
dye-phosphomolybdate reaction. The kit is stable for at least one year if handled and stored properly. The assay, compatible with microplate
formats, is simple and can be finished in 15 - 20 min. Reaction product is quantified by absorbance at 600-660 nm.
Kit Components:
Phosphate Assay Solution: 40 ml, store at 4ºC (for 400 assays)
NaH2PO4 Standard (10 mM): 1 ml, store at 4ºC
MSDS: Malachite Green, ammonium molybdate
Related kits: ATP assay
Product Description: Quantification of inorganic as well as organic phosphates (such as phospholipids, phosphoproteins, and nucleid acids) is of
interest in many biological studies. Phosphatase activity, for instance, can be derived from such an analysis after co-incubation of a phosphatase
and a specific phosphopeptide substrate (J. Cell. Biochem. 77:445,2000). Similarly, an assay for phospholipase activity can be performed after
hydrolysis of phospholipids by phospholipases. Although various methods can be employed to measure phosphates, non-radioactive colorimetric
protocols suitable for high-throughput analysis are the methods of choice in most laboratories. Perhaps the most sensitive colorimetric method for
phosphate assay is based on complex formation of phosphomolybdate with the basic dye Malachite Green (Anal. Biochem. 161:45,1987 &
271:29,1999). Our Phosphate Assay Kit (sufficient for 400 assays) is formulated to maximize the sensitivity and stability of the
dye-phosphomolybdate reaction. The kit is stable for at least one year if handled and stored properly. The assay, compatible with microplate
formats, is simple and can be finished in 15 - 20 min. Reaction product is quantified by absorbance at 600-660 nm.
Kit Components:
Phosphate Assay Solution: 40 ml, store at 4ºC (for 400 assays)
NaH2PO4 Standard (10 mM): 1 ml, store at 4ºC
MSDS: Malachite Green, ammonium molybdate
Related kits: ATP assay
 | |  | |  | |  | |  | |  | |  | |  | |  | |  | |  |
Biomedical Research Service
& Clinical Application
& Clinical Application
Normal (F1B strain) and cardiomyopathic
(TO2 strain) hamster hearts were
extracted with TCA. Concentrations of
inorganic phosphate were determined by
the Phosphate Assay Kit. The study
shows that the failing hamster heart
contains elevated levels of inorganic
phosphate, which may be associated with
increased alkaline phosphatase.
(TO2 strain) hamster hearts were
extracted with TCA. Concentrations of
inorganic phosphate were determined by
the Phosphate Assay Kit. The study
shows that the failing hamster heart
contains elevated levels of inorganic
phosphate, which may be associated with
increased alkaline phosphatase.