Phosphate Assay Kit                                                                                                                                                                                        Price

Product Description: Quantification of inorganic as well as organic phosphates (such as phospholipids, phosphoproteins, and nucleid acids) is
of interest in many biological studies. Phosphatase activity, for instance, can be derived from such an analysis after co-incubation of a
phosphatase and a specific phosphopeptide substrate (J. Cell. Biochem. 77:445,2000). Similarly, an assay for phospholipase activity can be
performed after hydrolysis of phospholipids by phospholipases. Although various methods can be employed to measure phosphates,
non-radioactive colorimetric protocols suitable for high-throughput analysis are the methods of choice in most laboratories. Perhaps the most
sensitive colorimetric method for phosphate assay is based on complex formation of phosphomolybdate with the basic dye Malachite Green (Anal.
Biochem. 161:45,1987 & 271:29,1999). Our Phosphate Assay Kit (sufficient for 400 assays) is formulated to maximize the sensitivity and stability
of the dye-phosphomolybdate reaction. The kit is stable for at least one year if handled and stored properly. The assay, compatible with
microplate formats, is simple and can be finished in 15 - 20 min. Reaction product is quantified by absorbance at 600-660 nm.

Kit Components:

Phosphate Assay Solution: 40 ml, store at 4ºC (for 400 assays)
NaH2PO4 Standard (10 mM): 1 ml, store at 4ºC

MSDS: Malachite Green, ammonium molybdate, HCl

Related kits: ATP assay

Citation:
Martin et al
Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts
Am J Physiol. 309:C139, 2015

Lin et al.
sFRP2 activates Wnt/beta-catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix
remodeling.
Am J. Physiol. 311:C710-C719, 2016
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Biomedical Research Service
& Clinical Application
Normal (F1B strain) and cardiomyopathic
(TO2 strain) hamster hearts were extracted
with TCA. Concentrations of inorganic
phosphate were determined by the
Phosphate Assay Kit. The study shows that
the failing hamster heart contains elevated
levels of inorganic phosphate, which may
be associated with increased alkaline
phosphatase.
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