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biomedical research biomedical research biomedical research biomedical research biomedical research biomedical
research plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA plasmid DNA adenovirus adenovirus
adenovirus adenovirus adenovirus adenovirus adenovirus lactate lactate lactate lactate lactate lactate lactate lactate
chemiluminescent chemiluminescent chemiluminescent chemiluminescent chemiluminescent TMB TMB TMB
chemiluminescent TMB TMB TMB TMB TMB TMB TMB genomic genomic genomic genomic genomic genomic RNA
RNA RNA RNA RNA RNA RNA RNA western blotting western blotting western blotting western blotting protein assay
protein assay protein assay protein assay protein assay SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
luciferase luciferase luciferase luciferase luciferase luciferase luciferase MTT MTT MTT MTT MTT MTT MTT LDH
LDH LDH LDH LDH LDH LDH cell injury cell injury cell injury cell injury cell injury cell proliferation cell proliferation
galactosidase galactosidase galactosidase galactosidase galactosidase galactosidase competent cell competent cell
competent cell competent cell biomedical research service biomedical research service biomedical research
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Protease Assay Kit
Product Description: The Protease Assay Kit (sufficient for 250 assays) offers a simple
non-radioactive method for routine assays monitoring the presence of contaminating
proteases in protein samples and following the isolation of proteases. Casein is used as
substrate in the assay kit, and its breakdown is measured by a simple colorimetric protein-dye
binding method. Degradation of casein by a protease causes a decrease in absorbance at
595 nm. The assay kit can also be custom modified for determining cofactor dependence, pH
or temperature optima, and potential regulation by a protease activator or inhibitor. For
instance, including 5 mM or 5 mM Ca2+ in the assay buffer allows one to determine the
activity of calpain I or calpain II, respectively. A protease control (trypsin) is included in the kit,
allowing users to perform a positive control assay. A set of 10x concentrated buffers from pH
6 - 8 are provided for determining pH vs. activity profiles of the proteases of interest. Assays
can be performed in microplates and measured by a microplate reader. Results can be
obtained in less than 30 min. Assay conditions for a particular protease can be easily
optimized for maximal sensitivity.
Biomedical Research Service
