ELISA Detection Kit/Horseradish Peroxidase (HRP)/TMB Detection System Price
Product Description: One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a
highly stable enzyme (Clin. Chim. Acta 81:1,1977). The compound 3,3',5,5'-tetramethylbenzidine (TMB) has emerged as the safest colorigenic
substrate of HRP exhibiting excellent sensitivity (Tetrahedron 30:3299,1974). HRP in the presence of peroxide converts TMB to a bluish reaction
product that can be read spectrophotometrically at 370 or 655 nm. The blue end product upon acidification by HCl or H2SO4 generates a bright
yellowish color enabling sensitive HRP detection and quantification by absorbance at 450 nm. This application has become widely used in
enzyme-linked immunosorbent assay (ELISA) because of its lowest detection limit compared to other reporter enzymes and substrate systems.
The working substrate solution needs to be prepared fresh by mixing TMB Substrate solution and TMB Peroxide solution. The ELISA Detection kit
is stable for ~1 year if stored and handled properly.The product is for research use only.
Kit Components:
TMB Substrate: 2 x 4 ml, store at 4ºC
TMB Peroxide: 100 ml, store at 4ºC
MSDS: TMB, Methanol, Hydrogen peroxide, Hydrochloric acid
Related kits: Enhanced Chemiluminescence (ECL) detection, Protein Assay Kit (EDTA/Mg2+ compatible)
Protein Assay Kit/D (detergent compatible)
Product Description: One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a
highly stable enzyme (Clin. Chim. Acta 81:1,1977). The compound 3,3',5,5'-tetramethylbenzidine (TMB) has emerged as the safest colorigenic
substrate of HRP exhibiting excellent sensitivity (Tetrahedron 30:3299,1974). HRP in the presence of peroxide converts TMB to a bluish reaction
product that can be read spectrophotometrically at 370 or 655 nm. The blue end product upon acidification by HCl or H2SO4 generates a bright
yellowish color enabling sensitive HRP detection and quantification by absorbance at 450 nm. This application has become widely used in
enzyme-linked immunosorbent assay (ELISA) because of its lowest detection limit compared to other reporter enzymes and substrate systems.
The working substrate solution needs to be prepared fresh by mixing TMB Substrate solution and TMB Peroxide solution. The ELISA Detection kit
is stable for ~1 year if stored and handled properly.The product is for research use only.
Kit Components:
TMB Substrate: 2 x 4 ml, store at 4ºC
TMB Peroxide: 100 ml, store at 4ºC
MSDS: TMB, Methanol, Hydrogen peroxide, Hydrochloric acid
Related kits: Enhanced Chemiluminescence (ECL) detection, Protein Assay Kit (EDTA/Mg2+ compatible)
Protein Assay Kit/D (detergent compatible)
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Biomedical Research Service
& Clinical Application
& Clinical Application
C2C12 myoblasts were treated with
saline (control) and 50 ng/ml interleukin-6
(IL-6) for 3 days. Culture media were
collected and assayed by Tumor
Necrosis Factor-alpha (TNF-alpha) ELISA
using an HRP-conjugated secondary
antibody. The ELISA Detection TMB
substrate solution was used for
quantification. The study shows that IL-6
caused downregulation of TNF-alpha in
cultured myoblasts, demonstrating an
anti-inflammatory property of IL-6.
saline (control) and 50 ng/ml interleukin-6
(IL-6) for 3 days. Culture media were
collected and assayed by Tumor
Necrosis Factor-alpha (TNF-alpha) ELISA
using an HRP-conjugated secondary
antibody. The ELISA Detection TMB
substrate solution was used for
quantification. The study shows that IL-6
caused downregulation of TNF-alpha in
cultured myoblasts, demonstrating an
anti-inflammatory property of IL-6.