Calpain Assay Kit Price
Product Description: Calpains are a family of cysteine proteases involved in the regulation of many protein substrates via limited proteolysis in
response to calcium signaling. This feature of calpains notably differs from proteasomes and autophagy, both of which degrade their protein
substrates. Two well-characterized members of the conventional calpain family in mammals are calpain 1 (mu isoform) and 2 (m isoform), which
are heterodimers of a specific 80-kDa large subunit and a common 28-kDa small subunit. Calpains have been implicated in numerous
calcium-regulated processes such as cell motility, cell cycle progression, cell death, and differentiation, making them ideal therapeutic targets.
The Calpain Assay Kit is derived from a modified colorimetric method (Arch Biochem Biophys 319:211,1995) for semi-quantitative analysis of
calpain activity in purified or crude samples. Casein is used as substrate in the assay kit and its breakdown is monitored by a protein-binding dye
present in Calpain Assay Solution. Sample calpain activity is measured by O.D.595nm. The kit is stable for at least one year under proper
storage and handling conditions.
Kit Components:
10x Cell Lysis Solution- 25 ml, store at 4ºC
Calpain Assay Solution- 20 ml, store at 4ºC (for 200 wells)
20mM CaCl2- 0.5 ml, store at 4ºC
10x Calpain Buffer- 0.5 ml, store at 4ºC
Casein Solution- 1 ml, store at -20ºC
0.1M DTT- 0.2 ml, store at -20ºC
MSDS: TX-100, DTT, Coomassie Brilliant Blue, hydrochloric acid, methanol
Related kits: MMP Assay, Cell Injury Assay, Peroxide Assay, Lipid Peroxidation Assay
Citation:
Walowitz et al
Proteolytic regulation of the zinc finger transcription factor YY1, a repressor of muscle-restricted gene expression
J. Biol. Chem. 273(12):6656-61, 1998
Product Description: Calpains are a family of cysteine proteases involved in the regulation of many protein substrates via limited proteolysis in
response to calcium signaling. This feature of calpains notably differs from proteasomes and autophagy, both of which degrade their protein
substrates. Two well-characterized members of the conventional calpain family in mammals are calpain 1 (mu isoform) and 2 (m isoform), which
are heterodimers of a specific 80-kDa large subunit and a common 28-kDa small subunit. Calpains have been implicated in numerous
calcium-regulated processes such as cell motility, cell cycle progression, cell death, and differentiation, making them ideal therapeutic targets.
The Calpain Assay Kit is derived from a modified colorimetric method (Arch Biochem Biophys 319:211,1995) for semi-quantitative analysis of
calpain activity in purified or crude samples. Casein is used as substrate in the assay kit and its breakdown is monitored by a protein-binding dye
present in Calpain Assay Solution. Sample calpain activity is measured by O.D.595nm. The kit is stable for at least one year under proper
storage and handling conditions.
Kit Components:
10x Cell Lysis Solution- 25 ml, store at 4ºC
Calpain Assay Solution- 20 ml, store at 4ºC (for 200 wells)
20mM CaCl2- 0.5 ml, store at 4ºC
10x Calpain Buffer- 0.5 ml, store at 4ºC
Casein Solution- 1 ml, store at -20ºC
0.1M DTT- 0.2 ml, store at -20ºC
MSDS: TX-100, DTT, Coomassie Brilliant Blue, hydrochloric acid, methanol
Related kits: MMP Assay, Cell Injury Assay, Peroxide Assay, Lipid Peroxidation Assay
Citation:
Walowitz et al
Proteolytic regulation of the zinc finger transcription factor YY1, a repressor of muscle-restricted gene expression
J. Biol. Chem. 273(12):6656-61, 1998
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Biomedical Research Service
& Clinical Application
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