Product Description:

In mammals, ethanol is metabolized mainly in the liver by alcohol dehydrogenase (ADH), which oxidizes ethanol to acetaldehyde. Acetaldehyde, a toxic metabolite responsible for the miserable effects of hangovers, is further oxidized to acetate by aldehyde dehydrogenase (ALDH). ALDH belongs to a large family of aldehyde dehydrogenases that can be found in many tissues of the body, but are at the highest concentrations in the liver. ALDH1 and ALDH2 display different abilities to detoxify small aldehydes (such as acetaldehyde) and large aldehydes (such as retinaldehyde). The assay kit uses a five-carbon aldehyde as ALDH substrate. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NADPH-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased ALDH activity. The enzyme assay solution should be kept at -80ºC, and will remain stable at -80ºC for ~1 year.

#Aldehyde Dehydrogenase​

Kit Components:

ALDH Assay Solution: 5 ml, store at -80ºC (for 100 wells)

10x ALDH Substrate: 0.5 ml, store at 4ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

glutaraldehyde, TX-100, DMSO, INT, Tris

Related Kits:

ADH Enzyme Assay, Ethanol Assay