Product Description: Price
Aldehyde dehydrogenases (ALDHs) are a superfamily of multiple isozymes that oxidize a wide range of aldehydes (aliphatic, aromatic, endogenous, and exogenous) to carboxylic acids using either NAD+ (more common) or NADP+ as a cofactor. Enzyme substrates for ALDHs can include formaldehyde, acetaldehyde, retinal, fatty aldehydes, and many aldehydes produced during drug metabolism and hepatic detoxification. Since ALDHs participate in many cellular functions, altered or dysregulated ALDHs can lead to accumulation of their substrates, which can damage the body. Indeed, dysfunctional ALDHs are associated with oncogenesis and pathogenesis in cardiovascular and neurological organ systems. ALDH nomenclature now spans from ALDH1 to ALDH24 across multiple species. Acetaldehyde dehydrogenase (ALDH2) is the key enzyme responsible for metabolism of the alcohol metabolite acetaldehyde in the liver. In particular, the involvement of ALDH2 expression in hepatitis, alcoholic liver disease, metabolic dysfunction-associated steatotic liver disease (MASLD), and cirrhosis have become clear. Thus, ALDH2 has emerged as a promising therapeutic target. The assay kit uses a five-carbon aldehyde (glutaraldehyde) as a stable ALDH substrate. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NAD- or NADP-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased ALDH activity. The enzyme assay solution should be kept at -80ºC, and will remain stable at -80ºC for many years. Select ALDH-NAD or ALDH-NADP Assay Kit.
#AldehydeDehydrogenase #ALDH #alcohol #aldehyde #ethanol
Kit Components:
ALDH-NAD or ALDH-NADP Assay Solution: 5 ml, store at -80ºC (for 100 wells)
10x ALDH Substrate: 0.5 ml, store at 4ºC
10x Sample Buffer: 25 ml, store at 4ºC
SDS:
glutaraldehyde, TX-100, DMSO, INT, Tris
Related Kits:
ADH Assay, Ethanol Assay
