Product Description: Price

alpha-Ketoglutarate (alpha-KG aka 2-oxoglutarate), the product of isocitrate dehydrogenase of the Krebs Cycle (TCA Cycle), represents a significant point of convergence in metabolism. Several amino acids can be converted to glutamate, which if transaminated or oxidatively deaminated, yields alpha-KG. Conversely, these amino acids can be synthesized from alpha-KG. Thus, intracellular levels of alpha- KG fluctuate according to nitrogen and carbon availability. As a Krebs cycle metabolite, it regulates anabolic and catabolic substrate availability as well as ATP production. In addition, it is an obligatory co-substrate for alpha-KG- and iron-dependent dioxygenases, which are a large group of phylogenetically conserved enzymes involved in hydroxylation reactions. As a substrate of the hydroxylases, alpha-KG exerts an impact on prolyl/aspartyl/lysyl hydroxylations, which in turn regulates the stability of the HIF-1 and collagen synthesis. Notably, alpha-KG binds and regulates G protein function because it is a ligand for GPR99/GPR80. The metabolic and extra-metabolic functions of alpha-KG thus offer many possibilities for therapeutic interventions. The alpha-KG Assay kit is based on the difference in the UV absorption spectra between the oxidized and reduced forms of NADP+/NADPH at 340 nm. As low as ~10 uM alpha-KG may be detected by the assay kit (assay range 10 – 200 uM). The assay requires a UV-transparent 96-well plate or a 0.2 ml Quartz cuvette for measuring changes in O.D.340 nm.

#alpha-Ketoglutarate

Kit Components:

alpha-KG Assay Solution: 10 ml (for 100 wells), store at -80ºC

GLDH (50x): 0.2 ml, store at -80ºC

NADPH (100x): 0.1 ml (brown vial), store at -80ºC

Ammonium (500x): 0.1 ml, store at -80ºC

20 mM alpha-KG standard: 0.2 ml, store at -80ºC

PEG Solution: 5 ml store at 4°C

MSDS:

Tris, ammonium chloride, PEG

Related Kits:

ATP Assay, Glutamine/Glutamate Assay, Pyruvate Assay