Product Description: Price

Alkaline phosphatase (ALP or ALK) possesses a hydrolytic activity removing phosphate groups from protein and non-protein molecules. ALP is present in all mammalian tissues, and multiple isozymes are differentially distributed in the body. While abnormal ALP levels can signal a wide range of clinical conditions, the primary importance of measuring ALP is diagnosis of bone or liver disease, although an elevated ALP usually requires other tests to determine the origin of the pathology. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) are two reporter enzymes widely used in immunoassays due to their stability. 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) is a synthetic chromogenic substrate, which upon cleavage by alkaline phosphatase is converted to 5-bromo-4-chloro-3-indole and inorganic phosphate. In the presence of a tetrazolium salt such as NBT or MTT, 5-bromo-4-chloro-3-indole is further converted to an insoluble dark blue diformazan precipitate, thus enabling its use for sensitive ALP-based protein detection. The working solution can be used for ALP-based in situ immunostaining and Western blotting. The kit is sufficient for preparing 200 ml of ALP staining solution.

#AlkalinePhosphatase #ALP #ALK #osteogenesis

Kit Components:

20x ALP Buffer: 10 ml, store at room temperature

50x BCIP: 4 ml, store at -20ºC

50x Tetrazolium: 4 ml, store at -20ºC

SDS:

BCIP, MTT, Tris, dimethylformamide, ethanol

Related Kits:

ALP Assay

Citation:

Martin et al

Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts

Am J Physiol Cell Physiol, 309:C139, 2015