Product Description: Price
The ammonium cation (NH4+) is formed by protonation of ammonia (NH3). Amino acid catabolism represents the major source of ammonia production in the human body. Amines obtained from the diet and monoamine neurotransmitters give rise to ammonia by the action of monoamine oxidase. In the catabolism of purines and pyrimidines, amino groups attached to the rings are released as ammonia. Ammonia is also formed from urea by the action of bacterial urease in the lumen of the intestine. Notably, ammonia is toxic if the blood concentration goes above 40 uM. The activity of the urea cycle is designed to keep the peripheral blood concentration of ammonia below the toxic level. While ammonia toxicity in the newborns is almost always due to genetic defects in the urea cycle, ammonia toxicity in adults usually results from liver damage due to alcohol, other poisons, or viral infection. The colorimetric assay kit is based on a modified Berthelot method for ammonia measurement and is suitable for biological samples, waste water, and soil samples. The reaction end product exhibits an absorption maximum at 660 nm. Assay sensitivity can be further enhanced by altering the reagent:sample ratio and volume if necessary. Assay reagents are stable for at least 1 year if stored and handled properly.
#Ammonia/ammonium
Kit Components:
Ammonia Assay Reagent 1: 10 ml (200 wells), store at 4ºC
Ammonia Assay Reagent 2: 2 ml, store at 4ºC
0.2 M NH4Cl standard: 0.1 ml, store at 4ºC
MSDS:
Sodium hypochlorite, Sodium Nitroprusside, Salicylic Acid, Ammonium Chloride
Related Kits:
Urea Assay, Glutamine/Glutamate Assay, MAO Assay
