Product Description: Price

Aldehyde oxidase (AO), a flavoenzyme structurally similar to xanthine oxidase (XO), functions in the biotransformation of many drugs and xenobiotics through oxidation of aldehydes and hydroxylation of N-heterocyclic compounds. The conversion of an aldehyde to an acid is accompanied by the production of hydrogen peroxide. The human AOX1 gene encodes the enzyme, which is highly concentrated in the liver and plays a vital role in the hepatic phase I metabolism, although some AO enzyme activity can also be detected in the lungs, kidneys, and intestines. The enzyme, possessing broad substrate specificity, thus contributes to the hepatic clearance of many compounds and drugs, and is receiving increasing attention in the field of pharmacokinetics. Control of AO-mediated metabolism, either by mitigation or utilization, is of significant pharmaceutical interest. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. Known inhibitors of AO include sterol and phenolic compounds such as estradiol, azopurine, cimetidine, and cyanide. The Aldehyde Oxidase Assay Kit uses glutaraldehyde as substrate for the production of hydrogen peroxide. Potassium iodide (KI) is used for colorimetric detection of hydrogen peroxide at 400-405 nm (molar extinction coefficient 5.3/mM-cm). Each kit is sufficient for ~50 samples using a 96-well format.

#AldehydeOxidase #drugmetabolism #pharmacokinetics

Kit Components:

AO Reaction: 0.5 ml, store at 4°C

AO Control: 0.5 ml, store at 4°C

Detection reagent: 15 g (100 wells), store at 4°C

10x Cell Lysis Solution: 25 ml, store at 4°C

SDS:

Tris, TX-100, EDTA, KI, glutaraldehyde

Related Kits:

Xanthine Dehydrogenase, MAO, Xanthine Oxidase