Product Description: Price

Asparagine synthetase, encoded by the ASNS gene, generates the amino acid asparagine from aspartate using glutamine as the nitrogen source and ATP as the energy source. The reaction also helps in the detoxification of ammonia within cells. The levels of ASNS mRNA increase in response to asparagine starvation as well as after leucine, isoleucine and glutamine deprivation. Genetic mutations in the ASNS gene can lead to a severe inherited disorder called Asparagine Synthetase Deficiency, which causes neurological problems like microcephaly, developmental delays, and seizures. Fifteen unique mutations in the ASNS gene have hitherto been clinically associated with asparagine synthetase deficiency. High levels of ASNS expression have been observed in circulating tumor cells, suggesting a role in metastasis. Notably, many acute lymphoblastic leukemia (ALL) cells exhibit little or no detectable expression of ASNS and are therefore sensitive to treatment by asparagine depletion. Further, pancreatic cancer cells overexpressing ASNS were found to exhibit increased resistance to apoptosis induced by cisplatin, suggesting clinical relevance of asparagine synthetase. The enzyme activity assay kit takes advantage of the glutaminase activity and relies on the detection of glutamate, which is coupled to the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which exhibits an absorption maximum at 492 nm.

#asparagine #ammonia #synthetase #amidotransferase #ASNS #ALL

Kit Components:

ASNS Buffer: 3 ml, store at -80°C

ASNS Substrate: 1 ml, store at -80°C

Glutamine: 100 mg, store at 4ºC

TA Assay Solution: 5 ml (100 wells), store at -80ºC
10x Cell Lysis Solution: 25 ml, store at RT

SDS:

DMSO, INT, Tris, EDTA, GLDH

Related Kits:
GAPDH Assay, GLS Assay, GDH Assay, AST Assay, ALT Assay, GFAT Assay