Product Description:

Aspartate aminotransferase (AST) is a member of the transaminase family of enzymes. The enzyme is previously referred to as glutamate oxaloacetate transaminase (GOT), which indicates its biological function in intermediary metabolism of glucose and protein through catalyzing the transfer of an amino group between aspartate and glutamate. AST is found in many tissues throughout the body, including the liver, heart, muscle, brain, and kidney, and therefore AST measurement along with ALT measurement is routinely performed in clinical diagnostics. While serum levels of AST are elevated in most types of liver disease, increased serum AST may also be associated with myocardial infarction, renal damage, and muscle necrosis. Assessment of the ratio of AST to ALT also provides clinically useful information. The colorimetric AST assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased AST activity. The kit is stable for at least one year if handled and stored properly.

Kit Components:

AST Substrate: 5 ml, store at -70°C

TA Assay Solution: 10 ml, store at -70°C (for 200 wells)

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, acetic acid, Hepes

Related Kits:

ALT Assay, HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme Assay

Citation:

Zhu et al

Enhanced anticancer activity of nanopreparation containing an MMP2-sensitive PEG-drug conjugate and cell-penetrating moiety

PNAS 110(42):17047-17052, 2013

Essex et al

Phospholipid-modified PEI-based nanocarriers for in vivo siRNA therapeutics against multidrug-resistant tumors

Gene Therapy 22:257-266, 2015