Product Description:

Intracellular ATP concentrations vary during cell growth and cell death. Notably, cells maintain a critical threshold of ATP levels, which dictates whether apoptotic or necrotic cell death may take place in conditions of stress. Evaluation of cellular ATP levels may thus provide useful information regarding the metabolic state and integrity of the cell. The enzyme luciferase catalyzes, in the presence of ATP, the oxidation of luciferin with concomitant emission of yellow-green light, which can be conveniently measured by a luminometer or scintillation counter. Light emission peaks in several seconds at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance and decay of the light flash requires consistent timing of the light measurement to obtain reliable data. This provides the basis for a sensitive non- radioactive ATP assay system capable of detecting 0.1 uM or 1 picomole of ATP and more convenient than that based on HPLC. The kit is sufficient for 200 assays using 0.1 ml of ATP Assay Solution per assay. The ATP Assay Solution if stored in aliquots at -80°C is stable for many years. Repeated freeze-thaw cycles should be avoided.

​#ATP

Kit Components:

ATP Assay Solution: 2 x 10 ml, store at -80°C (for 200 assays)

1 mM ATP Standard: 0.5 ml, store at -80°C

MSDS:

luciferin, EDTA, Tris, DTT

Related Kits:

Cell Proliferation Assay, Cell Injury Assay, ATP/ADP/AMP Assay, NAD+ Assay, Pyruvate Assay

Citation:

Dang et al

Hypoxia-inducible factor-1α promotes nonhypoxia-mediated proliferation in colon cancer cells and xenografts.

Cancer Research 66:1684-1693, 2006