Product Description:

Protein disulfide isomerase (PDI), a member of a large family of thio oxidoreductases, is a critical protein folding catalyst and molecular chaperone of the ER with an enriched expression in the pancreas and liver. Many mammalian ER-localized PDI homologs have been identified, including ERp18, ERp44, ERp57, ERp72, PDIR, etc, and E. coli also possesses PDI functional homologs. Although PDI has an ER retention sequence, the enzyme can be released from cells, directly binds to cell surface receptors, and influences their functions. The identification and development of small-molecule PDI inhibitors have been an active research area due to the involvement of PDI in disease. The PDI activity assay kit is based on an improved turbidometric assay using insulin as substrate in the presence of DTT. Solution turbidity in 96-well plate is monitored by optical density at 600-650 nm, which is proportional to PDI activity. The kit assays PDI activity present in crude cell/tissue lysates. The assay can be adapted to screen for small molecule inhibitors of PDI.

#Protein disulfide isomerase (PDI)

Kit Components:

PDI Solution-A: 10 ml (100 wells), store at 4°C

PDI Solution-B: 7 ml, store at room temperature

PDI Solution-C: 0.2 ml, store at 4°C

PDI Solution-D: 5 ml, store at room temperature

PDI Solution-E: 0.25 ml, store at 4°C

Dithiothreitol: 0.1 g, store at 4°C

10x Sample Buffer: 25 ml, store at 4°C

MSDS:

Tris, DTT, HCl