Product Description: Price

CTP synthase (CTPS1 and CTPS2) is an amidotransferase responsible for converting uridine triphosphate (UTP) to cytidine triphosphate (CTP) using glutamine as the nitrogen source. The CTPS enzymatic reaction constitutes the final and regulated step in de novo synthesis of pyrimidine nucleotides. The enzyme is a key player in cellular processes and is involved in development, cell proliferation, and immune response. Indeed, CTPS deficiency causes severe immunodeficiency and upregulated CTPS activity is widely seen in human and rodent tumors. Since CTPS is upregulated in rapidly proliferating cancer cells, it is an attractive target for drug development for cancer therapy. The balanced synthesis of pyrimidine nucleotides is crucial for synthesis of DNA/RNA and phospholipids. The tight regulation of CTPS is necessary to prevent nucleotide imbalances and support rapid cell proliferation. CTPS1 has a higher intrinsic enzymatic activity than CTPS2 and is particularly important during lymphocyte expansion and for cancer cell growth. Both isoforms can form filamentous structures called cytoophidia. The enzyme activity assay kit takes advantage of the glutaminase activity of CTPS and relies on the detection of glutamate, which is coupled to the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which exhibits an absorption maximum at 492 nm.

#pyrimidine #nucleotides #CTP synthase #de novo synthesis #UTP #CTP

Kit Components:

CTPS Buffer: 3 ml, store at -80°C

CTPS Substrate: 1 ml, store at -80°C

Glutamine: 100 mg, store at 4ºC

TA Assay Solution: 5 ml (100 wells), store at -80ºC
10x Cell Lysis Solution: 25 ml, store at RT

SDS:

DMSO, INT, Tris, EDTA, GLDH

Related Kits:
IMPDH Assay Kit, Xanthine Dehydrogenase Assay Kit, Adenylate Kinase Assay Kit, GMP Synthase Assay Kit