Product Description:

Cathepsin C (CTSC or DPP-1), an abundant lysosomal cysteine protease from the papain superfamily widely expressed in many mammalian tissues, is the only member of this protease family that consists of four identical subunits. In the acidic lysosomal milieu, CTSC functions as an amino dipeptidase cleaving depeptides from the N-terminus of its protein substrate. The enzyme is becoming recognized as one of the most versatile protease-processing system known so far, capable of proteolytic activation of granzymes A, B and C, cathepsin G, neutrophil elastase and chymase. CTSC-deficient mice fail to activate serine proteases from granules of cytotoxic T-lymphocytes, natural killer cells, neutrophils, and mast cells. LOF mutations in the gene result in early-onset periodontitis and palmoplantar keratosis, characteristics of Papillon– Lefevre syndrome (PLS). More than 50 mutations have been identified so far in PLS, all causing loss of CTSC function and activity, which is associated with subsequent severe reduction in the activity of neutrophil-derived serine proteases. The CTSC activity assay kit is based on proteolytic hydrolysis of the chromogenic peptide substrate Gly-Phe-p-nitroanilide (Anal Biochem 5:360, 1963). Cleavage of pNA from the substrate increases absorbance at 405 nm (extinction coefficient= 9.96 mM-1cm-1) and 410 nm (extinction coefficient= 8.8 mM-1cm-1), allowing for sensitive and quantitative assay of CTSC activity present in tissue/cell lysates. Kit components are stable for at least 1 year if stored and handled properly.

Kit Components:

10x CTSC Buffer: 0.6 ml, store at 4°C

10x Cell Lysis Solution: 25 ml, store at 4ºC

100x CTSC Substrate: 0.05 ml, store at -20°C (for 100 assays)

100x DTT: 0.1 ml, store at -20°C

SDS:

Sodium acetate, acetic acid, DTT, pNA, DMSO

Related Kits:

AAP Assay, CTSB Assay