BMR Enolase Assay Kit

Enolase, functioning in the second to the last glycolytic step, converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) while also capable of mediating the reverse direction during gluconeogenesis. Enolase 1, encoded by the ENO1 gene, is known as nonneuronal enolase (NNE) and is present in a variety of tissues. Enolase 2, the neuron-specific enolase (NSE) encoded by the ENO2 gene, is present at high levels in neural tissues. Enolase 3, or muscle specific enolase (MSE), is encoded by the ENO3. Enolase 1 is known to moonlight as a plasminogen receptor in the cell surface, contributing to cell proliferation, migration, and cancer metastasis. Overexpression of enolase 1 is observed in a broad range of cancers often associated with poor prognosis, which is usually accompanied by the presence of anti-ENO1 autoantibodies in some cancer patients. Enolase 2 plays a role in the pathogenesis of hypoxic-ischemic neuronal injury and can serve as a biomarker in ischemic brain damage. Elevated levels of enolase 2 are potentially toxic and may stimulate the expression of pro-inflammatory cytokines inducing neuronal cell death. Enolase 3 is an early marker of human myogenesis and could promote the progression of colorectal cancer by stimulating glycolysis. The Enolase Assay Kit is based on chemiluminescence measurement of ATP generated by combined actions of enolase and pyruvate kinase. The kit is stable for many years if stored and handled properly.

#enolase #glycolysis #ENO1 #ENO2 #ENO3 #2-phosphoglycerate #phosphoenolpyruvate

Kit Components:

Enolase Substrate: 0.15 ml, store at -80°C

PK Buffer: 0.5 ml, store at -80°C

PK Enzyme: 15 ul, store at 4°C

ATP Assay Solution: 10 ml (100 wells), store at -80°C

10x Cell Lysis Solution: 25 ml, store at 4°C

TCA Stop: 0.6 ml, store at 4°C

SDS:

luciferin, Tris, DTT, TCA, EDTA

Biomedical Research Service

Enolase Assay Kit