BMR Fatty Acid Oxidation Assay Kit

In tissues with high energy requirement, such as heart, up to 50 – 70% of energy comes from fatty acid beta-oxidation, which converts multiple types of fatty acids to acetyl-CoA with concomitant production of FADH2 and NADH in the mitochondria. This strictly aerobic oxidative pathway consists of four distinct steps: acyl-CoA dehydrogenation by acyl-CoA dehydrogenase, hydration by enoyl-CoA hydratase, dehydrogenation by 3-hydroxyacyl-CoA dehydrogenase, and thiolytic cleavage by a thiolase. The importance of the beta-oxidation system is exemplified by the existence of a group of genetic diseases categorized as fatty acid oxidation disorders (FAODs), which are a heterogeneous group of defects in fatty acid transport and oxidation. Measurement of FAO activity thus provides valuable insights into the pathophysiologic and metabolic basis of many diseases. The non-radioactive FAO assay is based on oxidation of palmitoyl-CoA (long chain), octanoyl-CoA (medium chain), or butyryl-CoA (short chain). Generation of NADH is coupled to the reduction of the tetrazolium salt INT to formazan. The intensity of the red-colored formazan is proportional to increased FAO activity. Please select E-141L (palmitoyl-CoA), E-141M (octanoyl-CoA), or E-141S (butyryl-CoA) for your order. E-141L, E-141M, and E-141S are used to assess the cell/tissue capacity for oxidation of long chain, medium chain, and short chain fatty acids, respectively.

#FattyAcidOxidation #FAO #beta-oxidation #fattyacid #acetyl-CoA #FAOD #palmiticacid #octanoicacid #butyricacid

E-141L Kit Components:

FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)

10x FAO Substrate (L): 0.5 ml, store at -80ºC

10x Sample Buffer: 25 ml, store at 4ºC

E-141M Kit Components:

FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)

20x FAO Substrate (M): 0.25 ml, store at -80ºC

10x Sample Buffer: 25 ml, store at 4ºC

E-141S Kit Components:

FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)

25x FAO Substrate (S): 0.2 ml, store at -80ºC